Anticorps Recombinant de lapin anti-CYFIP2

CYFIP2 Recombinant Antibody for IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

240909E3

N° de cat : 83845-3-RR

Synonymes

CYFIP1/2, 240909E3, p53-inducible protein 121, p53 inducible protein 121, KIAA1168

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Galerie de données de validation

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  IF Staining of Jurkat using 83845-3-RR

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using CYFIP2 antibody (83845-3-RR, Clone: 240909E3 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  FC experiment of Jurkat using 83845-3-RR

  1x10^6 Jurkat cells were intracellularly stained with 0.25 ug Cyfip2 Recombinant Antibody (83845-3-RR, Clone:240909E3) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 83845-3-RR

  Biolayer interferometry (BLl) kinetic assays of 83845-3-RR against Human CYFIP2 were performed. The affinity constant is 4.35 nM.

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Applications testées

• Résultats positifs en IF/ICC: cellules Jurkat,
• Résultats positifs en FC (Intra): cellules Jurkat,

Dilution recommandée

• Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

83845-3-RR cible CYFIP2 dans les applications de IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: CYFIP2 Protéine recombinante Ag8749
• Nom complet: cytoplasmic FMR1 interacting protein 2
• Masse moléculaire calculée: 148 kDa
• Numéro d’acquisition GenBank: BC011762
• Symbole du gène: CYFIP2
• Identification du gène (NCBI): 26999
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CYFIP1/2 belongs to the CYFIP family and is involved in T-cell adhesion and p53-dependent induction of apoptosis. CYFIP1 contains 1,253 amino acids and shares 88% sequence identity with CYFIP2. CYFIP1 and CYFIP2 are members of a highly conserved protein family and share approximately 99% sequence identity with their mouse orthologs. CYFIP1 has been shown to interact with the small GTPase RAC1, which is implicated in development and maintenance of neuronal structures. Consistent with FMRP and RAC1 localization in dendritic fine structures, CYFIP1/2 are present in synaptosomal extracts. This antibody can recognize both CYFIP1 and CYFIP2.

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