Anticorps Polyclonal de lapin anti-Cytoglobin

• Phare
• Validé par KD/KO

Cytoglobin Polyclonal Antibody for IHC, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

7

N° de cat : 13317-1-AP

Synonymes

CYGB, cytoglobin, HGB, Histoglobin, STAP

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Galerie de données de validation

• 

  WB analysis of mouse heart using 13317-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 13317-1-AP (Cytoglobin antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 13317-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 13317-1-AP (Cytoglobin antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse small intestine using 13317-1-AP

  mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 13317-1-AP (Cytoglobin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Transfected HEK-293 using 13317-1-AP

  Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 13317-1-AP (Cytoglobin antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse stomach using 13317-1-AP

  mouse stomach tissue were subjected to SDS PAGE followed by western blot with 13317-1-AP (Cytoglobin antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse small intestine using 13317-1-AP

  mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 13317-1-AP (Cytoglobin antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat kidney using 13317-1-AP

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 13317-1-AP (Cytoglobin antibody at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 13317-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 13317-1-AP (Cytoglobin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human uterus using 13317-1-AP

  human uterus tissue were subjected to SDS PAGE followed by western blot with 13317-1-AP (Cytoglobin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 13317-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 13317-1-AP (Cytoglobin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 13317-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 13317-1-AP (Cytoglobin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 13317-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 13317-1-AP (Cytoglobin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse spleen using 13317-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 13317-1-AP (Cytoglobin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 13317-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 13317-1-AP (Cytoglobin antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 13317-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 13317-1-AP (Cytoglobin antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human liver cancer using 13317-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 13317-1-AP (Cytoglobin antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human prostate cancer using 13317-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 13317-1-AP (Cytoglobin antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human prostate cancer using 13317-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 13317-1-AP (Cytoglobin antibody at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human heart using 13317-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 13317-1-AP (Cytoglobin antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 13317-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 13317-1-AP (Cytoglobin antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human heart using 13317-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 13317-1-AP (Cytoglobin antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 13317-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 13317-1-AP (Cytoglobin antibody at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human stomach using 13317-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 13317-1-AP (Cytoglobin antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human stomach using 13317-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 13317-1-AP (Cytoglobin antibody at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver using 13317-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 13317-1-AP (Cytoglobin antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 13317-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 13317-1-AP (Cytoglobin antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu cardiaque de souris, cellules HEK-293 transfectées, cellules PC-3, tissu cardiaque humain, tissu cérébral de souris, tissu d'estomac de souris, tissu d'intestin grêle de souris, tissu pulmonaire de souris, tissu rénal de rat, tissu rénal de souris, tissu splénique de souris, tissu utérin humain
• Résultats positifs en IHC: tissu de cancer du foie humain, tissu cardiaque humain, tissu de cancer de la prostate humain, tissu d'estomac humain, tissu hépatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 6 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

13317-1-AP cible Cytoglobin dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Cytoglobin Protéine recombinante Ag4161
• Nom complet: cytoglobin
• Masse moléculaire calculée: 190 aa, 21 kDa
• Poids moléculaire observé: 21-29 kDa
• Numéro d’acquisition GenBank: BC029798
• Symbole du gène: CYGB
• Identification du gène (NCBI): 114757
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Cytoglobin, also named as Histoglobin, HGb, STAP and CYGB, belongs to the globin family. Cytoglobin may have a protective function during conditions of oxidative stress. It may be involved in intracellular oxygen storage or transfer. It serves as a defensive mechanism against oxidative stress both in vitro and in vivo.(PMID:21224051) Cytoglobin is a ubiquitously expressed hexacoordinate hemoglobin that may facilitate diffusion of oxygen through tissues. The protein, with calculated MW of 21 kd, runs as a 29kd one in canine retina, kidney, liver, lung, and heart. It may be caused by posttranslational modification of the protein. Various immuno-staining patterns were reported including nuclear, cytoplasm and extracellular location.

Publications

Species	Application	Title
human,mouse,rat	WB,IF	Arterioscler Thromb Vasc Biol; The Hemoglobin Homolog Cytoglobin in Smooth Muscle Inhibits Apoptosis and Regulates Vascular Remodeling.; Authors - Frances L Jourd'heuil ; KD Validated
human	WB	Antioxid Redox Signal; The anti-oxidative role of cytoglobin in podocytes: implications for a role in chronic kidney disease.; Authors - Elisa B Randi
human	WB	Antioxidants (Basel); Cytoglobin Silencing Promotes Melanoma Malignancy but Sensitizes for Ferroptosis and Pyroptosis Therapy Response; Authors - Joey De Backer ; KD Validated
mouse	IHC	J Cell Physiol; Integrative proteomics and immunochemistry analysis of the factors in the necrosis and repair in acetaminophen-induced acute liver injury in mice.; Authors - Qin Feng
human	WB	Mol Med Rep; Intranasal administration of Cytoglobin modifies human umbilical cord‑derived mesenchymal stem cells and improves hypoxic‑ischemia brain damage in neonatal rats by modulating p38 MAPK signaling‑mediated apoptosis.; Authors - Hanhua Yang
human	WB	Vascul Pharmacol; Endothelial cell-induced cytoglobin expression in vascular smooth muscle cells contributes to modulation of nitric oxide.; Authors - Brenda Lilly