Anticorps Monoclonal anti-Cytoglobin

Cytoglobin Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(5)

CloneNo.

4C7E11

N° de cat : 60228-1-Ig

Synonymes

CYGB, 4C7E11, EC:1.11.1.-, EC:1.14.12.-, EC:1.15.1.1

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Galerie de données de validation

• 

  WB analysis of human heart using 60228-1-Ig

  human heart tissue were subjected to SDS PAGE followed by western blot with 60228-1-Ig (Cytoglobin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 60228-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60228-1-Ig (Cytoglobin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat heart using 60228-1-Ig

  rat heart tissue were subjected to SDS PAGE followed by western blot with 60228-1-Ig (Cytoglobin antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Transfected HEK-293 using 60228-1-Ig

  Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 60228-1-Ig (Cytoglobin antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig heart using 60228-1-Ig

  pig heart tissue were subjected to SDS PAGE followed by western blot with 60228-1-Ig (Cytoglobin antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig heart using 60228-1-Ig

  pig heart tissue were subjected to SDS PAGE followed by western blot with 60228-1-Ig (Cytoglobin antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver using 60228-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver using 60228-1-Ig(Cytoglobin antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 60228-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 60228-1-Ig (Cytoglobin antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 60228-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 60228-1-Ig (Cytoglobin antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 60228-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver using 60228-1-Ig(Cytoglobin antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human heart using 60228-1-Ig

  Immunohistochemical analysis of paraffin-embedded human heart using 60228-1-Ig(Cytoglobin antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 60228-1-Ig

  Immunohistochemical analysis of paraffin-embedded human heart using 60228-1-Ig(Cytoglobin antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human bladder using 60228-1-Ig

  Immunohistochemical analysis of paraffin-embedded human bladder using 60228-1-Ig(Cytoglobin antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human bladder using 60228-1-Ig

  Immunohistochemical analysis of paraffin-embedded human bladder using 60228-1-Ig(Cytoglobin antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 60228-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Cytoglobin antibody (60228-1-Ig, Clone: 4C7E11 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of HeLa using 60228-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Cytoglobin antibody (60228-1-Ig, Clone: 4C7E11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: Coeur humain, cellules HEK-293 transfectées, tissu cardiaque de lapin, tissu cardiaque de porc, tissu cardiaque humain
• Résultats positifs en IHC: tissu hépatique humain, tissu cardiaque humain, tissu vésical humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 5 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Informations sur le produit

60228-1-Ig cible Cytoglobin dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Cytoglobin Protéine recombinante Ag4161
• Nom complet: cytoglobin
• Masse moléculaire calculée: 190 aa, 21 kDa
• Poids moléculaire observé: 27-29 kDa
• Numéro d’acquisition GenBank: BC029798
• Symbole du gène: Cytoglobin
• Identification du gène (NCBI): 114757
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Cytoglobin is a ubiquitously expressed hexacoordinate hemoglobin that may facilitate diffusion of oxygen through tissues. The protein, with calculated MW of 21 kDa, runs as a 29 kDa one in canine retina, kidney, liver, lung, and heart. It may be caused by posttranslational modification of the protein. Various immuno-staining patterns were reported including nuclear, cytoplasm and extracellular location.

Publications

Species	Application	Title
human	WB	J Cell Mol Med; Cytoglobin promotes sensitivity to ferroptosis by regulating p53-YAP1 axis in colon cancer cells.; Authors - Shazhou Ye
human	WB,IP,IF	Mol Cell Biochem; Cytoglobin augments ferroptosis through autophagic degradation of ferritin in colorectal cancer cells; Authors - Chengjiang Fan
human	WB,IF	Cell Death Dis; ΔNp63α promotes Bortezomib resistance via the CYGB-ROS axis in head and neck squamous cell carcinoma.; Authors - Peng Zhou
mouse,human	WB	Mol Med Rep; Cytoglobin attenuates melanoma malignancy but protects melanoma cells from ferroptosis; Authors - Zuquan Zou
mouse	WB	Neural Regen Res; Treadmill exercise in combination with acousto-optic and olfactory stimulation improves cognitive function in APP/PS1 mice through the brain-derived neurotrophic factor- and Cygb-associated signaling pathways; Authors - Biao Xiao

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