Anticorps Polyclonal de lapin anti-CYP1A1

• Phare
• Validé par KD/KO

CYP1A1 Polyclonal Antibody for FC, IF, IHC, IP, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, porc, souris et plus (2)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

69

N° de cat : 13241-1-AP

Synonymes

AHH, AHRR, CP11, CYP1, CYP1A1, CYPIA1, Cytochrome P450 1A1, Cytochrome P450 C, Cytochrome P450 form 6, Cytochrome P450 P1, P1 450, P450 C, P450DX

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Galerie de données de validation

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  WB analysis of mouse cerebellum using 13241-1-AP

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 13241-1-AP (CYP1A1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 13241-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 13241-1-AP (CYP1A1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 13241-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 13241-1-AP (CYP1A1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 13241-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13241-1-AP (CYP1A1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 13241-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 13241-1-AP (CYP1A1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse lung using 13241-1-AP

  IP Result of anti-CYP1A1 (IP:13241-1-AP, 4ug; Detection:13241-1-AP 1:1000) with mouse lung tissue lysate 3600ug.

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  IHC staining of human breast cancer using 13241-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13241-1-AP (CYP1A1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human breast cancer using 13241-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13241-1-AP (CYP1A1 Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of HeLa using 13241-1-AP

  Immunofluorescent analysis of HeLa cells using 13241-1-AP (CYP1A1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of Jurkat using 13241-1-AP

  1X10^6 Jurkat cells were stained with 0.5ug CYP1A1 antibody (13241-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Applications testées

• Résultats positifs en WB: tissu de cervelet de souris, cellules HeLa, cellules HepG2, cellules K-562, tissu pulmonaire de souris
• Résultats positifs en IP: tissu pulmonaire de souris
• Résultats positifs en IHC: tissu de cancer du sein humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa
• Résultats positifs en cytométrie: cellules Jurkat

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:10-1:100
• Flow Cytometry (FC): FC : 0.50 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 58 publications below
• IHC: See 9 publications below
• IF: See 8 publications below

Informations sur le produit

13241-1-AP cible CYP1A1 dans les applications de WB, IP, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, porc, souris

• Réactivité: Humain, porc, souris
• Réactivité citée: rat, Humain, poisson-zèbre, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CYP1A1 Protéine recombinante Ag3990
• Nom complet: cytochrome P450, family 1, subfamily A, polypeptide 1
• Masse moléculaire calculée: 512 aa, 58 kDa
• Poids moléculaire observé: 50-58 kDa
• Numéro d’acquisition GenBank: BC023019
• Symbole du gène: CYP1A1
• Identification du gène (NCBI): 1543
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB,IF	Environ Int; Novel fluorescent and secreted transcriptional reporters for quantifying activity of the xenobiotic sensor aryl hydrocarbon receptor (AHR); Authors - Séverine A Degrelle
human,mouse	WB	Free Radic Biol Med; 5-Methoxyflavone ameliorates non-alcoholic fatty liver disease through targeting the cytochrome P450 1A1; Authors - Yurou Zhang
human,mouse	WB	Phytother Res; Quercetin ameliorates ulcerative colitis by activating aryl hydrocarbon receptor to improve intestinal barrier integrity; Authors - Xiaojing Wang
human	WB	Environ Pollut; Regioselective hydroxylation of carbendazim by mammalian cytochrome P450: A combined experimental and computational study.; Authors - Xia Lv
human	WB,IHC	BMC Med; NUPR1 contributes to radiation resistance by maintaining ROS homeostasis via AhR/CYP signal axis in hepatocellular carcinoma; Authors - Yizhi Zhan
human	WB,IF	J Gastroenterol; Ferroptosis in the colon epithelial cells as a therapeutic target for ulcerative colitis; Authors - Akihito Yokote