Anticorps Polyclonal de lapin anti-CYP1B1

CYP1B1 Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

14

Avis (1)

N° de cat : 18505-1-AP

Synonymes

CP1B, CYP1B1, CYPIB1, Cytochrome P450 1B1, GLC3A, P4501B1

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Galerie de données de validation

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  WB analysis using 18505-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18505-1-AP (CYP1B1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 18505-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 18505-1-AP (CYP1B1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 18505-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 18505-1-AP (CYP1B1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 18505-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 18505-1-AP (CYP1B1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 18505-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 18505-1-AP (CYP1B1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human colon cancer using 18505-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 18505-1-AP (CYP1B1 Antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules PC-3, cellules MCF-7, tissu cardiaque de souris, tissu cérébral de rat, tissu hépatique de rat
• Résultats positifs en IHC: tissu de cancer du côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 11 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Informations sur le produit

18505-1-AP cible CYP1B1 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CYP1B1 Protéine recombinante Ag13357
• Nom complet: cytochrome P450, family 1, subfamily B, polypeptide 1
• Masse moléculaire calculée: 61 kDa
• Poids moléculaire observé: 52 kDa
• Numéro d’acquisition GenBank: BC012049
• Symbole du gène: CYP1B1
• Identification du gène (NCBI): 1545
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Cytochrome P450 CYP1B1 is a recently cloned dioxin-inducible form of the cytochrome P450 family of xenobiotic metabolizing enzymes. CYP1B1 was found to be expressed at a high frequency in a wide range of human cancers of different histogenetic types, including cancers of the breast, colon, lung, esophagus, skin, lymph node, brain, and testis. There was no detectable immunostaining for CYP1B1 in normal tissues.(PMID:9230218). CYP1B1 is a 60 kDa protein that it also can be detected a band of 51 kDa or 57 kDa through the western blot(PMID:9525272; PMID: 9525272).

Publications

Species	Application	Title
human	WB	BMC Med; NUPR1 contributes to radiation resistance by maintaining ROS homeostasis via AhR/CYP signal axis in hepatocellular carcinoma; Authors - Yizhi Zhan
human	WB	PLoS Pathog; Reduced IRF4 expression promotes lytic phenotype in Type 2 EBV-infected B cells.; Authors - Jillian A Bristol
mouse	WB	Front Pharmacol; Astilbin Activates the Reactive Oxidative Species/PPARγ Pathway to Suppress Effector CD4+ T Cell Activities via Direct Binding With Cytochrome P450 1B1.; Authors - Shizhen Ding
human	WB	J Transl Med; piRNA-14633 promotes cervical cancer cell malignancy in a METTL14-dependent m6A RNA methylation manner.; Authors - Qi Xie
rat	WB	Sci Rep; PET-CT and RNA sequencing reveal novel targets for acupuncture-induced lowering of blood pressure in spontaneously hypertensive rats.; Authors - Jing Li
human	WB	J Dermatol Sci; Role of the aryl hydrocarbon receptor signaling pathway in promoting mitochondrial biogenesis against oxidative damage in human melanocytes.; Authors - Xiaowen Wang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Hala (Verified Customer) (11-23-2021)

works very good

• Applications: Western Blot
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: hepG2, hela cells