Anticorps Polyclonal de lapin anti-CYP24A1

• Phare
• Validé par KD/KO

CYP24A1 Polyclonal Antibody for IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, ELISA

Conjugaison

Non conjugué

Publications

13

Avis (1)

N° de cat : 21582-1-AP

Synonymes

24 OHase, CP24, CYP24, CYP24A1, Cytochrome P450 24A1, Cytochrome P450 CC24, P450 CC24, Vitamin D(3) 24 hydroxylase

---

Galerie de données de validation

• 

  WB analysis of A549 using 21582-1-AP

  WB result of CYP24A1 antibody (21582-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CYP24A1 transfected A549 cells.

• 

  WB analysis using 21582-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 21582-1-AP (CYP24A1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 21582-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 21582-1-AP (CYP24A1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 21582-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 21582-1-AP (CYP24A1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 21582-1-AP

  IP Result of anti-CYP24A1 (IP:21582-1-AP, 4ug; Detection:21582-1-AP 1:300) with HeLa cells lysate 2800ug.

• 

  IHC staining of human kidney using 21582-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 21582-1-AP (CYP24A1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 21582-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 21582-1-AP (CYP24A1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human prostate cancer using 21582-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 21582-1-AP (CYP24A1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human prostate cancer using 21582-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 21582-1-AP (CYP24A1 antibody) at dilution of 1:50 (under 40x lens).

---

Applications testées

• Résultats positifs en WB: cellules A549, cellules HeLa
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu rénal humain, tissu de cancer de la prostate humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 13 publications below
• IHC: See 3 publications below

Informations sur le produit

21582-1-AP cible CYP24A1 dans les applications de WB, IP, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CYP24A1 Protéine recombinante Ag16134
• Nom complet: cytochrome P450, family 24, subfamily A, polypeptide 1
• Masse moléculaire calculée: 514 aa, 59 kDa
• Poids moléculaire observé: 54-59 kDa
• Numéro d’acquisition GenBank: BC109083
• Symbole du gène: CYP24A1
• Identification du gène (NCBI): 1591
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CYP24A1(1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial) catalyzes the NADPH-dependent 24-hydroxylation of calcidiol (25-hydroxyvitamin D3) and 1-alpha,25-dihydroxyvitamin D3. Cholecalciferol is metabolised by the 25-hydroxylases (CYP2R1 and CYP27A1) in the liver, before 25-hydroxycholecalciferol (25(OH)D3) relocates to the circulation (PMID:20362668). The gene CYP24A1 has three isoforms, for the transit peptide "mitochondrial domain" removed, the MW of CYP24A1 will be 55-59 kDa, 47-51 kDa and 43 kDa. This antibody is specific to CYP24A1.

Publications

Species	Application	Title
human	WB,IHC	Cancer Lett; TAZ regulates cell proliferation and sensitivity to vitamin D3 in intrahepatic cholangiocarcinoma.; Authors - Heng Xiao ; KD Validated
mouse	WB	Acta Physiol (Oxf); Intestinal epithelial ablation of Pit-2/Slc20a2 in mice leads to sustained elevation of vitamin D3 upon dietary restriction of phosphate.; Authors - Eva Maria Pastor-Arroyo
mouse	WB	J Bone Miner Res; Exercise May Ameliorate the Detrimental Side Effects of High Vitamin D Supplementation on Muscle Function in Mice.; Authors - Danielle A Debruin
human	WB	Cancer Commun (Lond); Squalene epoxidase promotes colorectal cancer cell proliferation through accumulating calcitriol and activating CYP24A1-mediated MAPK signaling.; Authors - Luwei He
human	WB, IHC	J Steroid Biochem Mol Biol; Eldecalcitol (ED-71), an Analog of 1α, 25-dihydroxy Vitamin D3 as a Potential Anti-cancer Agent for Oral Squamous Cell Carcinomas.; Authors - T Shintani
mouse	WB	J Physiol; 1,25(OH)2 vitamin D3 stimulates active phosphate transport but not paracellular phosphate absorption in mouse intestine.; Authors - Nati Hernando

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

Kenzo (Verified Customer) (08-30-2023)

This antibody appeared to work pretty well for immunofluorescence in mouse tissues.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Kidney