Anticorps Monoclonal anti-CYP27A1

• Phare
• Validé par KD/KO

CYP27A1 Monoclonal Antibody for WB, IHC, IF-P, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF-P, ELISA

Conjugaison

Non conjugué

Publications(6)

CloneNo.

3G3F7

N° de cat : 67045-1-Ig

Synonymes

3G3F7, 5-beta-cholestane-3-alpha,7-alpha,12-alpha-triol 26-hydroxylase, CP27, CTX, CYP27

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Galerie de données de validation

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  WB analysis of HepG2 using 67045-1-Ig

  WB result of CYP27A1 antibody (67045-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CYP27A1 transfected HepG2 cells.

• 

  WB analysis of Caco-2 using 67045-1-Ig

  Caco-2 cells were subjected to SDS PAGE followed by western blot with 67045-1-Ig (CYP27A1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat liver using 67045-1-Ig

  rat liver tissue were subjected to SDS PAGE followed by western blot with 67045-1-Ig (CYP27A1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 67045-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 67045-1-Ig (CYP27A1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HT-29 using 67045-1-Ig

  HT-29 cells were subjected to SDS PAGE followed by western blot with 67045-1-Ig (CYP27A1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 67045-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 67045-1-Ig (CYP27A1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HT-29 using 67045-1-Ig

  HT-29 cells were subjected to SDS PAGE followed by western blot with 67045-1-Ig (CYP27A1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 67045-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67045-1-Ig (CYP27A1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 67045-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67045-1-Ig (CYP27A1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human liver cancer using 67045-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CYP27A1 antibody (67045-1-Ig, Clone: 3G3F7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of human liver cancer using 67045-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CYP27A1 antibody (67045-1-Ig, Clone: 3G3F7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules Caco-2, cellules HepG2, cellules HT-29, tissu hépatique de rat
• Résultats positifs en IHC: tissu de cancer du foie humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu de cancer du foie humain,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochimie (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 6 publications below
• IF: See 1 publications below

Informations sur le produit

67045-1-Ig cible CYP27A1 dans les applications de WB, IHC, IF-P, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: CYP27A1 Protéine recombinante Ag7746
• Nom complet: cytochrome P450, family 27, subfamily A, polypeptide 1
• Masse moléculaire calculée: 60 kDa
• Poids moléculaire observé: 54-60 kDa
• Numéro d’acquisition GenBank: BC051851
• Symbole du gène: CYP27A1
• Identification du gène (NCBI): 1593
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB	J Bone Miner Res; CYP4A22 Loss-of-Function Causes A New Type of Vitamin D-dependent Rickets (VDDR1C); Authors - Xiaohong Duan
mouse	WB	J Ethnopharmacol; Penthorum chinense Pursh. extract attenuates non-alcholic fatty liver disease by regulating gut microbiota and bile acid metabolism in mice.; Authors - Xiaoxi Li
mouse	WB	Nat Microbiol; Akkermansia muciniphila protects mice against an emerging tick-borne viral pathogen; Authors - Jinyan Xie
human	WB	Front Immunol; Construction of a lipid metabolism-related risk model for hepatocellular carcinoma by single cell and machine learning analysis; Authors - Lisha Mou
mouse	WB	Adv Sci (Weinh); Geniposidic Acid Targeting FXR "S332 and H447" Mediated Conformational Change to Upregulate CYPs and miR-19a-3p to Ameliorate Drug-Induced Liver Injury; Authors - Minqi Fan
rat	WB,IF	J Ethnopharmacol; Integration of network pharmacology, UHPLC-Q exactive orbitrap HRMS technique and metabolomics to elucidate the active ingredients and mechanisms of compound danshen pills in treating hypercholesterolemic rats; Authors - Shanlan Li

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