Anticorps Polyclonal de lapin anti-CYP2J2

CYP2J2 Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

5

N° de cat : 13562-1-AP

Synonymes

Arachidonic acid epoxygenase, CPJ2, CYP2J2, CYPIIJ2, Cytochrome P450 2J2

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Galerie de données de validation

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  WB analysis using 13562-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13562-1-AP (CYP2J2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 13562-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13562-1-AP (CYP2J2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 13562-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 13562-1-AP (CYP2J2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 13562-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 13562-1-AP (CYP2J2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver cancer using 13562-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 13562-1-AP (CYP2J2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of mouse heart using 13562-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 13562-1-AP (CYP2J2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 13562-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 13562-1-AP (CYP2J2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 13562-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 13562-1-AP (CYP2J2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 13562-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 13562-1-AP (CYP2J2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human heart using 13562-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 13562-1-AP (CYP2J2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 13562-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 13562-1-AP (CYP2J2 antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules HUVEC, cellules HepG2, cellules L02, tissu cardiaque de rat, tissu cardiaque de souris, tissu de muscle squelettique de souris
• Résultats positifs en IHC: tissu hépatique humain, tissu cardiaque de souris, tissu cardiaque humain, tissu de cancer du foie humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Informations sur le produit

13562-1-AP cible CYP2J2 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CYP2J2 Protéine recombinante Ag4435
• Nom complet: cytochrome P450, family 2, subfamily J, polypeptide 2
• Masse moléculaire calculée: 502 aa, 58 kDa
• Poids moléculaire observé: 58-65 kDa
• Numéro d’acquisition GenBank: BC032594
• Symbole du gène: CYP2J2
• Identification du gène (NCBI): 1573
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CYP2J2(Cytochrome P450 2J2) belongs to the cytochrome P450 family. This enzyme metabolizes arachidonic acid predominantly via a NADPH-dependent olefin epoxidation to all four regioisomeric cis-epoxyeicosatrienoic acids. The human CYP2J member, CYP2J2, is shown to be expressed in several tissues such as the small intestine, liver, heart, kidney, and lung and may play a role in the oxidative bioactivation of arachidonic acid(PMID:12386130).

Publications

Species	Application	Title
human	IHC	J Hematol Oncol; Integrated proteogenomic characterization of urothelial carcinoma of the bladder.; Authors - Ning Xu
rat	WB,IHC,IF	Life Sci; CYP2J2-produced epoxyeicosatrienoic acids attenuate ischemia/reperfusion-induced acute kidney injury by activating the SIRT1-FoxO3a pathway.; Authors - Ye Zhu
human	WB	Pharmacology; Berberine Induces CYP2J2 Expression in Human U251 Glioma Cells via Regulation of Peroxisome Proliferator-Activated Receptor Alpha.; Authors - Xuming Yu
		PeerJ; CYP2C11 played a significant role in down-regulating rat blood pressure under the challenge of a high-salt diet.; Authors - Wei Liu
human	WB	Placenta; Altered distribution of fatty acid exerting lipid metabolism and transport at the maternal-fetal interface in fetal growth restriction; Authors - Zhongmei Yang