Anticorps Monoclonal anti-CYP3A4

CYP3A4 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Conjugaison

Non conjugué

Publications(4)

CloneNo.

2F11C4

N° de cat : 67110-1-Ig

Synonymes

2F11C4, Cholesterol 25-hydroxylase, CP33, CP34, CYP3A

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Galerie de données de validation

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  WB analysis of mouse liver using 67110-1-Ig

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 67110-1-Ig (CYP3A4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 67110-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67110-1-Ig (CYP3A4 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig liver using 67110-1-Ig

  pig liver tissue were subjected to SDS PAGE followed by western blot with 67110-1-Ig (CYP3A4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig gall bladder using 67110-1-Ig

  pig gall bladder tissue were subjected to SDS PAGE followed by western blot with 67110-1-Ig (CYP3A4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 67110-1-Ig

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 67110-1-Ig (CYP3A4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse liver using 67110-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 67110-1-Ig (CYP3A4 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 67110-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 67110-1-Ig (CYP3A4 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 67110-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67110-1-Ig (CYP3A4 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 67110-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67110-1-Ig (CYP3A4 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse liver using 67110-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using CYP3A4 antibody (67110-1-Ig, Clone: 2F11C4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of mouse liver using 67110-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using CYP3A4 antibody (67110-1-Ig, Clone: 2F11C4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of rat liver using 67110-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed rat liver tissue using CYP3A4 antibody (67110-1-Ig, Clone: 2F11C4 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of HepG2 using 67110-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CYP3A4 antibody (67110-1-Ig, Clone: 2F11C4 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

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Applications testées

• Résultats positifs en WB: tissu hépatique de souris, tissu hépatique de porc, tissu hépatique de rat, tissu pulmonaire de souris
• Résultats positifs en IHC: tissu hépatique de souris, tissu de cancer du foie humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu hépatique de souris, tissu hépatique de rat
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:300-1:1200
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IF: See 3 publications below

Informations sur le produit

67110-1-Ig cible CYP3A4 dans les applications de WB, IHC, IF/ICC, IF-P, ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: CYP3A4 Protéine recombinante Ag13074
• Nom complet: cytochrome P450, family 3, subfamily A, polypeptide 4
• Masse moléculaire calculée: 503 aa, 57 kDa
• Poids moléculaire observé: 51-57 kDa
• Numéro d’acquisition GenBank: BC101631
• Symbole du gène: CYP3A4
• Identification du gène (NCBI): 1576
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
mouse	WB	Biomaterials; Hepatic endoplasmic reticulum-derived nanodiscs for broad-spectrum drug detoxification; Authors - Lei Sun
human	IF	Biomed Rep; Phenotypical, functional and transcriptomic comparison of two modified methods of hepatocyte differentiation from human induced pluripotent stem cells.; Authors - Rong Li
human	IF	Methods Mol Biol; Homogeneous Differentiation of Functional Hepatocytes from Human Induced Pluripotent Stem Cells.; Authors - Rong Li
human	IF	Acta Biomater; Hepar-on-a-sensor-platform with hybridization chain reaction amplification strategy to intuitively monitor the hepatoxicity of natural compounds; Authors - Wenhui Qian

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