Anticorps Polyclonal de lapin anti-CYR61/CCN1

• Phare
• Validé par KD/KO

CYR61/CCN1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (2)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugaison

Non conjugué

Publications(44)

N° de cat : 26689-1-AP

Synonymes

CYR61, CCN family member 1, CCN1, Cellular communication network factor 1, Cysteine-rich angiogenic inducer 61

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Galerie de données de validation

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  WB analysis using 26689-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26689-1-AP (CYR61/CCN1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of MDA-MB-453s using 26689-1-AP

  MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 26689-1-AP (CYR61/CCN1 antibody at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 26689-1-AP

  IP result of anti-CYR61/CCN1 (IP:26689-1-AP, 4ug; Detection:26689-1-AP 1:800) with HeLa cells lysate 1200 ug.

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  IHC staining of human colon cancer using 26689-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 26689-1-AP (CYR61/CCN1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 26689-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 26689-1-AP (CYR61/CCN1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 26689-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 26689-1-AP (CYR61/CCN1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A431 using 26689-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using CYR61/CCN1 antibody (26689-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  IF Staining of MCF-7 using 26689-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using CYR61/CCN1 antibody (26689-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  FC experiment of HeLa using 26689-1-AP

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug CYR61/CCN1 Polyclonal antibody (26689-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules A431, cellules HeLa, cellules MDA-MB-453s
• Résultats positifs en IP: cellules HeLa,
• Résultats positifs en IHC: tissu de cancer du côlon humain, tissu de tumeur ovarienne humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules A431, cellules MCF-7
• Résultats positifs en FC (Intra): cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 4 publications below
• WB: See 39 publications below
• IHC: See 10 publications below
• IF: See 10 publications below
• IP: See 1 publications below

Informations sur le produit

26689-1-AP cible CYR61/CCN1 dans les applications de WB, IHC, IF/ICC, FC (Intra), IP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CYR61/CCN1 Protéine recombinante Ag25129
• Nom complet: cysteine-rich, angiogenic inducer, 61
• Masse moléculaire calculée: 42 kDa
• Poids moléculaire observé: 42 kDa
• Numéro d’acquisition GenBank: BC001271
• Symbole du gène: CYR61
• Identification du gène (NCBI): 3491
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CYR61 is a member of the CYR61/CTGF/NOV (CCN) protein family. CYR61 participated in cell mitogenesis, cellular adhesion, migration, differentiation, angiogenesis, and survival. Cyr61 is especially associated with diseases related to chronic inflammation, including RA, Crohn's disease and ulcerative colitis. CYR61 may serve essential roles as either an oncogene or a tumor suppressor, depending on the cancer cell type. CYR61 also induces angiogenesis, which supplies oxygen and nutrients to tumors during growth. CYR61 can also play a role in the proliferation, invasion, survival, and metastasis of cancer cells.

Publications

Species	Application	Title
human,mouse	WB,IF	Nat Commun; A translational program that suppresses metabolism to shield the genome.; Authors - Nathan C Balukoff
human,mouse	WB,IF	Dev Cell; Mitochondrial CCN1 drives ferroptosis via fatty acid β-oxidation; Authors - Wanxin Guo
human	WB	EMBO J; MST2 methylation by PRMT5 inhibits Hippo signaling and promotes pancreatic cancer progression; Authors - Yan Sun
		ACS Appl Mater Interfaces; A Novel Electrochemiluminescent Immunoassay Based on Target Transformation Assisted with Catalyzed Hairpin Assembly Amplification for the Ultrasensitive Bioassay.; Authors - Hu-Xin Lei
human	WB	Acta Pharmacol Sin; Urolithin A promotes atherosclerotic plaque stability by limiting inflammation and hypercholesteremia in Apolipoprotein E-deficient mice; Authors - Meng-Yun Xu
human	WB	Mol Ther Nucleic Acids; The RNA binding protein QKI5 suppresses ovarian cancer via downregulating transcriptional coactivator TAZ.; Authors - Tao Liu

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