Anticorps Polyclonal de lapin anti-MT-CYB

• Phare
• Validé par KD/KO

MT-CYB Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (2)

Applications

WB, IF, ELISA

Conjugaison

Non conjugué

Publications(115)

Avis (3)

N° de cat : 55090-1-AP

Synonymes

CYTB, COB, Complex III subunit 3, Complex III subunit III, MT CYB

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Galerie de données de validation

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  WB analysis using 55090-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55090-1-AP (CYTB antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HepG2, cellules MDA-MB-231

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 112 publications below
• IF: See 5 publications below

Informations sur le produit

55090-1-AP cible MT-CYB dans les applications de WB, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: cytochrome b
• Masse moléculaire calculée: 43 kDa
• Poids moléculaire observé: 43 kDa
• Numéro d’acquisition GenBank: YP_003024038
• Symbole du gène: CYTB
• Identification du gène (NCBI): 4519
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CYTB, also named as COB and MTCYB, is a component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is a respiratory chain that generates an electrochemical potential coupled to ATP synthesis. It's mitochondrion membrane protein with MW 43 kDa.

Publications

Species	Application	Title
human	WB	Cell Metab; Malic enzyme 2 connects the Krebs cycle intermediate fumarate to mitochondrial biogenesis.; Authors - Yi-Ping Wang
human	WB	Mol Cell; Hepatic micropeptide modulates mitochondrial RNA processing machinery in hepatocellular carcinoma; Authors - Linyu Zhu
human	WB	Nucleic Acids Res; Selection of initiator tRNA and start codon by mammalian mitochondrial initiation factor 3 in leaderless mRNA translation; Authors - Muhoon Lee
human	WB	Cell Rep Med; End binding-3 inhibitor activates regenerative program in age-related macular degeneration; Authors - Quinn Lee
human	WB	Nat Commun; RNA modification landscape of the human mitochondrial tRNALys regulates protein synthesis.; Authors - Uwe Richter
mouse	WB	Nat Commun; Mitofusin 1 and 2 regulation of mitochondrial DNA content is a critical determinant of glucose homeostasis.; Authors - Vaibhav Sidarala

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Baptiste (Verified Customer) (05-23-2025)

Working well but have a light unspecific band

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Lymphoblastoid

MT-CYB Antibody Western Blot validation (1:1000 dilution) in Lymphoblastoid (Cat no:55090-1-AP)

FH

Yan (Verified Customer) (10-21-2024)

Worked fine in WB assay. Perhaps I should've used more concentrated.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Human primary fibroblasts

MT-CYB Antibody Western Blot validation (1:1000 dilution) in Human primary fibroblasts (Cat no:55090-1-AP)

FH

Mi (Verified Customer) (02-10-2023)

Works very well in differentiated human white adipocyte cells.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: adipocytes

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