Anticorps Polyclonal de lapin anti-Cytohesin 2

• Phare
• Validé par KD/KO

Cytohesin 2 Polyclonal Antibody for IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

4

N° de cat : 10405-1-AP

Synonymes

ARF exchange factor, ARNO, CTS18, CTS18.1, CYTH2, cytohesin 2, Protein ARNO, PSCD2, PSCD2L, SEC7L, Sec7p L, Sec7p like

---

Galerie de données de validation

• 

  WB analysis of mouse heart using 10405-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 10405-1-AP (Cytohesin 2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 10405-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 10405-1-AP (Cytohesin 2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse heart using 10405-1-AP

  IP Result of anti-Cytohesin 2 (IP:10405-1-AP, 4ug; Detection:10405-1-AP 1:500) with mouse heart tissue lysate 4000ug.

• 

  IHC staining of human colon using 10405-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10405-1-AP (Cytohesin 2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 10405-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10405-1-AP (Cytohesin 2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach using 10405-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 10405-1-AP (Cytohesin 2 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human stomach using 10405-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 10405-1-AP (Cytohesin 2 antibody) at dilution of 1:200 (under 40x lens).

---

Applications testées

• Résultats positifs en WB: tissu cardiaque de souris,
• Résultats positifs en IP: tissu cardiaque de souris
• Résultats positifs en IHC: tissu de côlon humain, tissu d'estomac humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Informations sur le produit

10405-1-AP cible Cytohesin 2 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Cytohesin 2 Protéine recombinante Ag0687
• Nom complet: cytohesin 2
• Masse moléculaire calculée: 47 kDa
• Poids moléculaire observé: 47 kDa
• Numéro d’acquisition GenBank: BC004361
• Symbole du gène: CYTH2
• Identification du gène (NCBI): 9266
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Pleckstrin homology, Sec7 and coiled/coil domains 2 (PSCD2) is a member of the PSCD family. Members of this family have identical structural organization that consists of an N-terminal coiled-coil motif, a central Sec7 domain, and a C-terminal pleckstrin homology (PH) domain. The coiled-coil motif is involved in homodimerization, the Sec7 domain contains guanine-nucleotide exchange protein (GEP) activity, and the PH domain interacts with phospholipids and is responsible for association of PSCDs with membranes. Members of this family appear to mediate the regulation of protein sorting and membrane trafficking. PSCD2 exhibits GEP activity in vitro with small G proteins ARF1, ARF3, and ARF6.

Publications

Species	Application	Title
rat	WB, IF	J Neurosci; Inhibition of Cytohesins Protects against Genetic Models of Motor Neuron Disease.; Authors - Jinbin Zhai
mouse	WB,IHC	J Biol Chem; The synaptic scaffolding protein CNKSR2 interacts with CYTH2 to mediate hippocampal granule cell development.; Authors - Hidenori Ito ; KD Validated
human	WB	Oncol Rep; Cytohesin-2 as a novel prognostic marker for hepatocellular carcinoma.; Authors - Xu Kedong K
rat	WB,IP	Open Biol; ARNO is recruited by the neuronal adaptor FE65 to potentiate ARF6-mediated neurite outgrowth; Authors - Yuqi Zhai