Anticorps Polyclonal de lapin anti-Calponin

Calponin Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Conjugaison

Non conjugué

Publications(14)

N° de cat : 24855-1-AP

Synonymes

CNN1, CNN 1, Calponin-1, Calponin 1, Basic calponin

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Galerie de données de validation

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  WB analysis of mouse stomach using 24855-1-AP

  mouse stomach tissue were subjected to SDS PAGE followed by western blot with 24855-1-AP (Calponin Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon using 24855-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 24855-1-AP (Calponin antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human heart using 24855-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 24855-1-AP (Calponin Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human heart using 24855-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 24855-1-AP (Calponin Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human breast hyperplasia using 24855-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 24855-1-AP (Calponin Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human skeletal muscle using 24855-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 24855-1-AP (Calponin Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human skeletal muscle using 24855-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 24855-1-AP (Calponin Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of mouse heart using 24855-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using Calponin antibody (24855-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of C2C12 using 24855-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed C2C12 cells using Calponin antibody (24855-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu d'estomac de souris
• Résultats positifs en IHC: tissu de côlon humain, tissu cardiaque humain, tissu de muscle squelettique humain, tissu d'hyperplasie mammaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu cardiaque de souris,
• Résultats positifs en IF/ICC: cellules C2C12,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochimie (IHC): IHC : 1:300-1:1200
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 14 publications below

Informations sur le produit

24855-1-AP cible Calponin dans les applications de WB, IHC, IF/ICC, IF-P, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Calponin Protéine recombinante Ag21384
• Nom complet: calponin 1, basic, smooth muscle
• Masse moléculaire calculée: 33 kDa
• Poids moléculaire observé: 34 kDa
• Numéro d’acquisition GenBank: BC022015
• Symbole du gène: Calponin
• Identification du gène (NCBI): 1264
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Calponin is a family of actin filament-associated proteins which regulate smooth muscle cell contraction. Three isoforms of calponin exist: calponin h1 (CNN1) , calponin h2 (CNN2) and calponin 3 (CNN3). CNN1 is a basic 34-kDa protein specifically expressed in smooth muscle and a marker of smooth muscle cell differentiation. This antibody raised against the specific region of human CNN1, thus it does not cross-react with CNN2 or CNN3.

Publications

Species	Application	Title
human	WB	Bioact Mater; A TEMPOL and rapamycin loaded nanofiber-covered stent favors endothelialization and mitigates neointimal hyperplasia and local inflammation.; Authors - Rui Wang
human	WB	Mol Ther Nucleic Acids; Long Non-coding RNA PEBP1P2 Suppresses Proliferative VSMCs Phenotypic Switching and Proliferation in Atherosclerosis.; Authors - Xingqiang He
rat	WB	J Ginseng Res; Protective effect and mechanism of ginsenoside Rg2 on atherosclerosis; Authors - Qianqian Xue
mouse	WB	Sci Rep; Nicotine facilitates VSMC dysfunction through a miR-200b/RhoGDIA/cytoskeleton module.; Authors - Dongli Liang
human,rat	WB	Front Mol Biosci; Integrated Strategies of Diverse Feature Selection Methods Identify Aging-Based Reliable Gene Signatures for Ischemic Cardiomyopathy.; Authors - Huafeng Song
human	WB	J Mol Cell Cardiol; miR-564: A potential regulator of vascular smooth muscle cells and therapeutic target for aortic dissection.; Authors - Min Li

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