Anticorps Polyclonal de lapin anti-calreticulin

• Phare
• Validé par KD/KO

calreticulin Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

Publications(73)

N° de cat : 27298-1-AP

Synonymes

CALR, Calregulin, CRT

---

Galerie de données de validation

• 

  WB analysis using 27298-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27298-1-AP (calreticulin antibody) at dilution of 1:60000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 27298-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27298-1-AP (Calreticulin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 27298-1-AP

  WB result of Calreticulin antibody (27298-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Calreticulin transfected HeLa cells.

• 

  WB analysis using 27298-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27298-1-AP (Calreticulin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 27298-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 27298-1-AP (calreticulin Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human thyroid using 27298-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid tissue slide using 27298-1-AP (calreticulin Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human thyroid using 27298-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid tissue slide using 27298-1-AP (calreticulin Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human colon using 27298-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27298-1-AP (calreticulin antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 27298-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 27298-1-AP (calreticulin antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 27298-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Calreticulin antibody (27298-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of HeLa using 27298-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Calreticulin antibody (27298-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidine (red).

• 

  IF Staining of HepG2 using 27298-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using calreticulin antibody (27298-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

• 

  IF Staining of SKOV-3 using 27298-1-AP

  Immunofluorescent analysis of (4% PFA) fixed SKOV-3 cells using Calreticulin antibody (27298-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HeLa using 27298-1-AP

  1x10^6 HeLa cells were intracellularly stained with 0.4 ug calreticulin Polyclonal antibody (27298-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

---

Applications testées

• Résultats positifs en WB: cellules HeLa, cellules C6, cellules NIH/3T3, cellules SH-SY5Y, tissu cérébral de rat, tissu cérébral de souris, tissu de muscle squelettique de souris
• Résultats positifs en IHC: tissu thyroïdien humain, human normal colon; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2, cellules HeLa, cellules SKOV-3
• Résultats positifs en FC (Intra): cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:20000-1:100000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 27 publications below
• IHC: See 3 publications below
• IF: See 45 publications below

Informations sur le produit

27298-1-AP cible calreticulin dans les applications de WB, IHC, IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, singe, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: calreticulin Protéine recombinante Ag26064
• Nom complet: calreticulin
• Masse moléculaire calculée: 60 kDa
• Poids moléculaire observé: 55 kDa
• Numéro d’acquisition GenBank: BC002500
• Symbole du gène: Calreticulin
• Identification du gène (NCBI): 811
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CALR,also named as grp60, ERp60, HACBP, CRP55, CRTC and Calregulin, belongs to the calreticulin family. It is a molecular calcium-binding chaperone promoting folding, oligomeric assembly and quality control in the ER via the calreticulin/calnexin cycle. CALR is a ER marker. It interacts transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER. CALR interacts with the DNA-binding domain of NR3C1 and mediates its nuclear export. The MW of CALR migrates aberrantly at 60 kD by SDS-PAGE. Some study provided that it's a new possibility for CRT-mediated tumor immune prevention and treatment.

Publications

Species	Application	Title
human	WB	Cell Metab; Transient Receptor Potential V Channels Are Essential for Glucose Sensing by Aldolase and AMPK.; Authors - Mengqi Li
mouse	IF	Nat Commun; In situ generation of micrometer-sized tumor cell-derived vesicles as autologous cancer vaccines for boosting systemic immune responses; Authors - Yuxin Guo
mouse	IF	Nat Commun; Engineered macrophages as near-infrared light activated drug vectors for chemo-photodynamic therapy of primary and bone metastatic breast cancer.; Authors - Yanjuan Huang
human	WB	EMBO J; STING directly recruits WIPI2 for autophagosome formation during STING-induced autophagy; Authors - Wei Wan
mouse		J Control Release; Positron emission tomography guided synergistic treatment of melanoma using multifunctional zirconium-hematoporphyrin nanosonosensitizers; Authors - Xiaodan Jiao
mouse,human	WB,IF	ACS Appl Mater Interfaces; A Versatile Nanoplatform for Broad-Spectrum Immunotherapy by Reversing the Tumor Microenvironment; Authors - Ao Chen

{{ptg:RelatedPrimaryAntibodies}}