Anticorps Recombinant de lapin anti-Caspase 1

• Phare
• Validé par KD/KO

Caspase 1 Recombinant Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (2)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications(40)

CloneNo.

1O18

N° de cat : 81482-1-RR

Synonymes

CASP1, Caspase1, 1O18, CASP 1, CASP-1

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Galerie de données de validation

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  WB analysis using 81482-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 81482-1-RR (Caspase 1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 81482-1-RR

  Biolayer interferometry (BLl) kinetic assays of 81482-1-RR against Human Caspase 1 were performed. The affinity constant is 305 nM.

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Applications testées

• Résultats positifs en WB: cellules Raji, cellules THP-1, cellules U-937

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 39 publications below
• IHC: See 2 publications below
• IF: See 4 publications below

Informations sur le produit

81482-1-RR cible Caspase 1 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Caspase 1 Protéine recombinante Ag11143
• Nom complet: caspase 1, apoptosis-related cysteine peptidase (interleukin 1, beta, convertase)
• Masse moléculaire calculée: 404 aa, 45 kDa
• Poids moléculaire observé: 30 kDa, 35 kDa, 43-45 kDa
• Numéro d’acquisition GenBank: BC062327
• Symbole du gène: Caspase 1
• Identification du gène (NCBI): 834
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CASP1(caspase-1) is also named as IL1BC, IL1BCE and belongs to the peptidase C14A family. It is a cysteine protease that regulates inflammatory processes through its capacity to process and activate the interleukin-1-beta (IL1B), IL18, and IL33 precursor proteins. The active caspase-1 can increase cellular membrane permeability and intracellular calcium levels, which facilitates lysosome exocytosis and release of host antimicrobial factors and microbial products (PMID:21804020). It has 5 isoforms produced by alternative splicing. 81482-1-RR can recognize the 45 kDa Pro-Caspase1 and the 30-35 kDa splicing isoforms or active dimer form of caspase 1.

Publications

Species	Application	Title
human	WB	Adv Sci (Weinh); Scutellarin Rescued Mitochondrial Damage through Ameliorating Mitochondrial Glucose Oxidation via the Pdk-Pdc Axis; Authors - Ning Sheng
rat	WB	Phytomedicine; Artesunate ameliorates diabetic xerostomia in rats through regulating oral microbiota and metabolic profile in salivary gland based on NF-κB/NLRP3 signaling pathway; Authors - Siqin Zhang
rat	WB,IF	Phytomedicine; Systemic pharmacology reveal the mechanism by which the Qiangjin Zhuanggu Qufeng mixture inhibits LPS-induced pyroptosis of rat nucleus pulposus cells; Authors - Dong Wang
mouse	WB	Int Immunopharmacol; Triggering receptor expressed on myeloid cells-1 aggravates obliterative bronchiolitis via enhancing the proinflammatory phenotype of macrophages; Authors - Junhao Wan
mouse	WB,IF	J Zhejiang Univ Sci B; Roles of PANoptosis and related genes in acute liver failure: neoteric insight from bioinformatics analysis and animal experiment verification; Authors - Tiantian Ge
mouse	WB	Antioxidants (Basel); Bisdemethoxycurcumin Alleviates Dextran Sodium Sulfate-Induced Colitis via Inhibiting NLRP3 Inflammasome Activation and Modulating the Gut Microbiota in Mice; Authors - Jingfei Zhang

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