Anticorps Polyclonal de lapin anti-Cathepsin L

• Phare
• Validé par KD/KO

Cathepsin L Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications(10)

N° de cat : 27952-1-AP

Synonymes

Cathepsin L, cathepsin L1, CATL, CTSL, CTSL1, FLJ31037, Major excreted protein, MEP

---

Galerie de données de validation

• 

  WB analysis of A549 using 27952-1-AP

  WB result of Cathepsin L antibody (27952-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-Cathepsin L transfected A549 cells.

• 

  WB analysis using 27952-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 27952-1-AP (Cathepsin L antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 27952-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 27952-1-AP (Cathepsin L antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 27952-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 27952-1-AP (Cathepsin L antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Applications testées

• Résultats positifs en WB: cellules A549,
• Résultats positifs en IHC: tissu de cancer du foie humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 8 publications below
• IHC: See 4 publications below
• IF: See 2 publications below

Informations sur le produit

27952-1-AP cible Cathepsin L dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Cathepsin L Protéine recombinante Ag27813
• Nom complet: cathepsin L1
• Masse moléculaire calculée: 38 kDa
• Poids moléculaire observé: 36-39 kDa, 29 kDa
• Numéro d’acquisition GenBank: BC012612
• Symbole du gène: Cathepsin L
• Identification du gène (NCBI): 1514
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CTSL1(Cathepsin L1) is also named as CTSL and MEP, belongs to the peptidase C1 family. It is a lysosomal proteinase whose expression is also up-regulated in the skeletal muscle during starvation(PMID:20088826). It plays an intracellular role in normal intestinal epithelial polarization and initiation of neoplasia(PMID:17622569). CTSL1 also improves cardiac function and inhibits cardiac hypertrophy, inflammation, and fibrosis through blocking AKT/GSK3B signaling(PMID:19096818). The full length protein is 38 kDa with a signal peptide, two propeptide and a glycosylation site. It has been detected the 36 kDa, 39 kDa, 29 kDa and 21 kDa in rat gastrocnemius muscle. These forms of cathepsin L could either be attributed to differences in glycosylation or to partial processing of the proenzyme. (PMID:11696001)

Publications

Species	Application	Title
mouse	IHC	Front Pharmacol; Nodosin Exerts an Anti-Colorectal Cancer Effect by Inhibiting Proliferation and Triggering Complex Cell Death in Vitro and in Vivo; Authors - Huixia Fan
human	WB	Acta Pharmacol Sin; Salvianolic acid B suppresses hepatic fibrosis by inhibiting ceramide glucosyltransferase in hepatic stellate cells; Authors - Zi-Bo Li
human	IHC	Transl Oncol; Mitochondria-related chemoradiotherapy resistance genes-based machine learning model associated with immune cell infiltration on the prognosis of esophageal cancer and its value in pan-cancer; Authors - Ziyu Liu
mouse	WB	J Med Chem; Design, Synthesis, and Biological Evaluation of 2-Arylaminopyrimidine Derivatives as Dual Cathepsin L and JAK Inhibitors for the Treatment of Acute Lung Injury; Authors - Chunwei Shen
human	WB	Pharmacol Res; The molecular receptor NKBB enhances the persistence and anti-hepatocellular carcinoma activity of GPC3 CAR-T cells; Authors - Minghao Sui
human	WB,IHC,IF	World J Gastroenterol; Macrophage-derived cathepsin L promotes epithelial-mesenchymal transition and M2 polarization in gastric cancer; Authors - Lu-Xi Xiao ; KD Validated

{{ptg:RelatedPrimaryAntibodies}}