Anticorps Monoclonal anti-Cathepsin L

• Phare
• Validé par KD/KO

Cathepsin L Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(5)

CloneNo.

1A12G8

N° de cat : 66914-1-Ig

Synonymes

CTSL1, 1A12G8, Cathepsin L heavy chain, Cathepsin L light chain, cathepsin L1

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Galerie de données de validation

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  WB analysis of A549 using 66914-1-Ig

  A549 cells were subjected to SDS PAGE followed by western blot with 66914-1-Ig (Cathepsin L antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 66914-1-Ig

  WB result of Cathepsin L antibody (66914-1-Ig; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Cathepsin L transfected A549 cells.

• 

  WB analysis of NCI-H1299 using 66914-1-Ig

  NCI-H1299 cells were subjected to SDS PAGE followed by western blot with 66914-1-Ig (Cathepsin L antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HUVEC using 66914-1-Ig

  HUVEC cells were subjected to SDS PAGE followed by western blot with 66914-1-Ig (Cathepsin L antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 66914-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66914-1-Ig (Cathepsin L antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 66914-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66914-1-Ig (Cathepsin L antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human prostate cancer using 66914-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66914-1-Ig (Cathepsin L antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HEK-293 using 66914-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using Cathepsin L antibody (66914-1-Ig, Clone: 1A12G8 ) at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1).

• 

  IF Staining of HEK-293 using 66914-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using Cathepsin L antibody (66914-1-Ig, Clone: 1A12G8 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

• 

  Affinity and Kinetic Characterization of 66914-1-Ig

  Biolayer interferometry (BLl) kinetic assay of 66914-1-Ig against Human Cathepsin L was performed. The affinity constant is 4.84 nM.

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Applications testées

• Résultats positifs en WB: cellules A549, cellules HeLa, cellules HepG2, cellules HUVEC, cellules NCI-H1299
• Résultats positifs en IHC: tissu de cancer de la prostate humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HEK-293

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 1 publications below

Informations sur le produit

66914-1-Ig cible Cathepsin L dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Cathepsin L Protéine recombinante Ag27951
• Nom complet: cathepsin L1
• Masse moléculaire calculée: 38 kDa
• Poids moléculaire observé: 43 kDa
• Numéro d’acquisition GenBank: BC012612
• Symbole du gène: Cathepsin L
• Identification du gène (NCBI): 1514
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CTSL1(Cathepsin L1) is also named as CTSL and MEP, belongs to the peptidase C1 family. It is a lysosomal proteinase whose expression is also up-regulated in the skeletal muscle during starvation(PMID:20088826). It plays an intracellular role in normal intestinal epithelial polarization and initiation of neoplasia(PMID:17622569). CTSL1 also improves cardiac function and inhibits cardiac hypertrophy, inflammation, and fibrosis through blocking AKT/GSK3B signaling(PMID:19096818). The full length protein is 38 kDa with a signal peptide, two propeptide and a glycosylation site. It has been detected the 36 kDa, 39 kDa, 29 kDa and 21 kDa in rat gastrocnemius muscle. These forms of cathepsin L could either be attributed to differences in glycosylation or to partial processing of the proenzyme. (PMID:11696001)

Publications

Species	Application	Title
human	WB	Mol Cancer; Sulindac (K-80003) with nab-paclitaxel and gemcitabine overcomes drug-resistant pancreatic cancer; Authors - Cheng-Ke Xie
	WB	Clin Sci (Lond); Loss of FoxO1 activates an alternate mechanism of mitochondrial quality control for healthy adipose browning; Authors - Limin Shi
	IHC	Tissue Cell; High expression of SERPINE1 and CTSL in keratinocytes in pressure injury caused by ischemia-reperfusion injury; Authors - Lexuan Bi
human	WB	Cancer Sci; Methyltransferase-like 3 promotes cervical cancer metastasis by enhancing cathepsin L mRNA stability in an N6-methyladenosine-dependent manner; Authors - Pingping Liu ; KD Validated
human	WB	Chem Biodivers; Design, Synthesis and Biological Evaluation of Naphthalimide-Polyamine Conjugate as a Potential Anti-Colorectal Cancer Agent; Authors - Fujun Dai

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