Anticorps Monoclonal anti-Caveolin-1

• Phare
• Validé par KD/KO

Caveolin-1 Monoclonal Antibody for FC (Intra), IF

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat, souris

Applications

IF, FC (Intra)

Conjugaison

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

6C2B2

N° de cat : CL488-66067

Synonymes

BSCL3, CAV, CAV1, Caveolin 1, Caveolin-1, CGL3, MSTP085, VIP21

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Galerie de données de validation

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  IF Staining of human skin cancer using CL488-66067

  Immunofluorescent analysis of (4% PFA) fixed human skin cancer tissue using CL488-66067 (Caveolin-1 antibody) at dilution of 1:100.

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  IF Staining of human liver cancer using CL488-66067

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CL488-66067 (Caveolin-1 antibody) at dilution of 1:100.

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  IF Staining of human liver cancer using CL488-66067

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CL488-66067 (Caveolin-1 antibody) at dilution of 1:100.

• 

  IF Staining of human liver cancer using CL488-66067

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CL488-66067 (Caveolin-1 antibody) at dilution of 1:100.

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  IF Staining of human liver cancer using CL488-66067

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CL488-66067 (Caveolin-1 antibody) at dilution of 1:100.

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  FC experiment of HeLa using CL488-66067

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human Caveolin-1 (CL488-66067, Clone:6C2B2) (red), or 0.4 ug Mouse IgG1 Isotype Control (CL488-66360, Clone: T1F8D3F10) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en IF: tissu de cancer de la peau humain, tissu de cancer du foie humain
• Résultats positifs en cytométrie: cellules HeLa,

Dilution recommandée

• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

CL488-66067 cible Caveolin-1 dans les applications de IF, FC (Intra) et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Caveolin-1 Protéine recombinante Ag8049
• Nom complet: caveolin 1, caveolae protein, 22kDa
• Masse moléculaire calculée: 22 kDa
• Poids moléculaire observé: 20-25 kDa
• Numéro d’acquisition GenBank: BC006432
• Symbole du gène: CAV1
• Identification du gène (NCBI): 857
• Conjugaison: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec glycérol à 50 %, Proclin300 à 0,05 % et BSA à 0,5 %, pH 7,3.
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Caveolin-1 (CAV1), a multifunctional protein, is the main constituent molecule of caveolae and represents a scaffolding molecule for several signaling molecules including epidermal growth factor receptor (PMID: 19641024). Several studies have implicated that a reduced expression of CAV1 was found in cancers including head and neck carcinoma (PMID: 19002186). However, other studies recognize CAV1 as a tumor promoter because CAV1 is overexpressed in various kinds of cancers, especially in oral cancer (PMID: 20558341). Recent study also show that CAV1 is involved in gastric Cancer (PMID: 25339030).

Produits associés

Flow Cytometry Perm Buffer (10X)

PF00011-C