Anticorps Monoclonal anti-Caveolin-1

• Phare
• Validé par KD/KO

Caveolin-1 Monoclonal Antibody for IF-P, FC (Intra)

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat, souris

Applications

IF-P, FC (Intra)

Conjugaison

CoraLite®594 Fluorescent Dye

Publications(1)

CloneNo.

6C2B2

N° de cat : CL594-66067

Synonymes

Caveolin 1, CAV1, CGL3, CAV, BSCL3

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Galerie de données de validation

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  IF Staining of human skin cancer using CL594-66067

  Immunofluorescent analysis of (4% PFA) fixed human skin cancer tissue using CL594-66067 (Caveolin-1 antibody) at dilution of 1:100.

• 

  IF Staining of human skin cancer using CL594-66067

  Immunofluorescent analysis of (4% PFA) fixed human skin cancer tissue using CL594-66067 (Caveolin-1 antibody) at dilution of 1:100.

• 

  IF Staining of human liver cancer using CL594-66067

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CoraLite®594 Caveolin-1 antibody (CL594-66067, Clone: 6C2B2 ) at dilution of 1:200.

• 

  IF Staining of human liver cancer using CL594-66067

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CoraLite®594 Caveolin-1 antibody (CL594-66067, Clone: 6C2B2 ) at dilution of 1:200.

• 

  IF Staining of human liver cancer using CL594-66067

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CoraLite®594 Caveolin-1 antibody (CL594-66067, Clone: 6C2B2 ) at dilution of 1:200.

• 

  IF Staining of human liver cancer using CL594-66067

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CL594-66067 (Caveolin-1 antibody) at dilution of 1:100.

• 

  IF Staining of human liver cancer using CL594-66067

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CL594-66067 (Caveolin-1 antibody) at dilution of 1:100.

• 

  IF Staining of human liver cancer using CL594-66067

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CL594-66067 (Caveolin-1 antibody) at dilution of 1:100.

• 

  IF Staining of human liver cancer using CL594-66067

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CL594-66067 (Caveolin-1 antibody) at dilution of 1:100.

• 

  FC experiment of HeLa using CL594-66067

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human Caveolin-1 (CL594-66067, Clone:6C2B2) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en IF-P: tissu de cancer du foie humain, tissu de cancer de la peau humain
• Résultats positifs en FC (Intra): cellules HeLa,

Dilution recommandée

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IF: See 1 publications below

Informations sur le produit

CL594-66067 cible Caveolin-1 dans les applications de IF-P, FC (Intra) et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Réactivité citée: souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Caveolin-1 Protéine recombinante Ag8049
• Nom complet: caveolin 1, caveolae protein, 22kDa
• Masse moléculaire calculée: 22 kDa
• Poids moléculaire observé: 20-25 kDa
• Numéro d’acquisition GenBank: BC006432
• Symbole du gène: CAV1
• Identification du gène (NCBI): 857
• Conjugaison: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Caveolin-1 (CAV1), a multifunctional protein, is the main constituent molecule of caveolae and represents a scaffolding molecule for several signaling molecules including epidermal growth factor receptor (PMID: 19641024). Several studies have implicated that a reduced expression of CAV1 was found in cancers including head and neck carcinoma (PMID: 19002186). However, other studies recognize CAV1 as a tumor promoter because CAV1 is overexpressed in various kinds of cancers, especially in oral cancer (PMID: 20558341). Recent study also show that CAV1 is involved in gastric cancer (PMID: 25339030).

Publications

Species	Application	Title
mouse	IF	Mutat Res; Downregulated CAV-1 in mouse spinal cord may alleviate bone cancer pain by inhibiting the ERK/CREB pathway; Authors - Jianyun Ge

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