Anticorps Polyclonal de lapin anti-CD2AP

CD2AP Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications(4)

N° de cat : 24122-1-AP

Synonymes

METS-1, Mets1, METS 1, Mesenchyme-to-epithelium transition protein with SH3 domains 1, CD2-associated protein

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Galerie de données de validation

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  WB analysis using 24122-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24122-1-AP (Cd2ap antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 24122-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 24122-1-AP (Cd2ap antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse kidney using 24122-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 24122-1-AP (Cd2ap antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 24122-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 24122-1-AP (Cd2ap antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse stomach using 24122-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 24122-1-AP (Cd2ap antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse stomach using 24122-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 24122-1-AP (Cd2ap antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 24122-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 24122-1-AP (Cd2ap antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 24122-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 24122-1-AP (Cd2ap antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules K-562, tissu rénal de souris
• Résultats positifs en IHC: tissu rénal de souris, tissu d'estomac de souris, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:12000
• Immunohistochimie (IHC): IHC : 1:400-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below
• IHC: See 1 publications below

Informations sur le produit

24122-1-AP cible CD2AP dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CD2AP Protéine recombinante Ag21170
• Nom complet: CD2-associated protein
• Masse moléculaire calculée: 637 aa, 70 kDa
• Poids moléculaire observé: 74 kDa
• Numéro d’acquisition GenBank: BC157901
• Symbole du gène: Cd2ap
• Identification du gène (NCBI): 12488
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CD2 associated protein (CD2AP, also known as CMS) is a scaffolding molecule that acts as an adapter protein between membrane proteins and the actin cytoskeleton (PMID: 10339567). CD2AP also plays a role in epithelial cell junction formation and affects the pathogenesis of Alzheimer's disease (PMID: 22891260; 31440393). Its mutations are associated with sporadic nephrotic syndrome and focal segmental glomerulosclerosis (FSGS) (PMID: 17713465; 19131354).

Publications

Species	Application	Title
mouse	WB,IHC	Sci Rep; Disulfidptosis links the pathophysiology of ulcerative colitis and immune infiltration in colon adenocarcinoma; Authors - Chenhao Jiang
human	WB	Front Med (Lausanne); MCC Regulator of WNT Signaling Pathway (MCC) Is a Podocyte Essential Gene.; Authors - Hui Song
mouse	WB	Am J Physiol Cell Physiol; Long non-coding RNA Kcnq1ot1 promotes sC5b-9-induced podocyte pyroptosis by inhibiting miR-486a-3p and upregulating NLRP3.; Authors - Chunjian Zhang
mouse	WB	Inflammation; Fractalkine is Involved in Lipopolysaccharide-Induced Podocyte Injury through the Wnt/β-Catenin Pathway in an Acute Kidney Injury Mouse Model.; Authors - Soulixay Senouthai

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