Anticorps Polyclonal de lapin anti-CD2AP

• Phare
• Validé par KD/KO

CD2AP Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IF/ICC, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications(9)

N° de cat : 51046-1-AP

Synonymes

METS-1, Mets1, METS 1, Mesenchyme-to-epithelium transition protein with SH3 domains 1, CD2-associated protein

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Galerie de données de validation

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  WB analysis of mouse kidney using 51046-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 51046-1-AP (Cd2ap antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 51046-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 51046-1-AP (Cd2ap Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 51046-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 51046-1-AP (Cd2ap Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 51046-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 51046-1-AP (Cd2ap antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 51046-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 51046-1-AP (Cd2ap Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 51046-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 51046-1-AP (Cd2ap antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 51046-1-AP

  IP result of anti-Cd2ap (IP:51046-1-AP, 4ug; Detection:51046-1-AP 1:2000) with mouse brain tissue lysate 2560ug.

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  IP experiment of HeLa cells using 51046-1-AP

  IP result of anti-Cd2AP (51046-1-AP for IP and Detection).

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  IF Staining of HepG2 using 51046-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 51046-1-AP (Cd2ap antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of A431 using 51046-1-AP

  Immunofluorescent analysis of A431 cells using 51046-1-AP (Cd2ap antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu rénal de souris, cellules A431, cellules HeLa, cellules Jurkat, tissu cérébral de souris, tissu testiculaire de souris
• Résultats positifs en IP: tissu cérébral de souris, cellules HeLa
• Résultats positifs en IF/ICC: cellules HepG2, cellules A431

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:20000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 8 publications below
• IF: See 4 publications below
• CoIP: See 1 publications below

Informations sur le produit

51046-1-AP cible CD2AP dans les applications de WB, IF/ICC, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CD2AP Protéine recombinante Ag0622
• Nom complet: CD2-associated protein
• Masse moléculaire calculée: 637 aa, 70 kDa
• Poids moléculaire observé: 71 kDa
• Numéro d’acquisition GenBank: BC157901
• Symbole du gène: Cd2ap
• Identification du gène (NCBI): 12488
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CD2 associated protein (CD2AP, also known as CMS) is a scaffolding molecule that acts as an adapter protein between membrane proteins and the actin cytoskeleton (PMID: 10339567). CD2AP also plays a role in epithelial cell junction formation and affects the pathogenesis of Alzheimer's disease (PMID: 22891260; 31440393). Its mutations are associated with sporadic nephrotic syndrome and focal segmental glomerulosclerosis (FSGS) (PMID: 17713465; 19131354).

Publications

Species	Application	Title
mouse	IF	J Clin Invest; Endoplasmic reticulum-associated degradation is required for nephrin maturation and kidney glomerular filtration function.; Authors - Sei Yoshida
human	WB	Exp Ther Med; Transforming growth factor-β1-induced podocyte injury is associated with increased microRNA-155 expression, enhanced inflammatory responses and MAPK pathway activation.; Authors - Xintong Zheng
human	WB,IF	J Biol Chem; The Rab GTPase-binding protein EHBP1L1 and its interactors CD2AP/CIN85 negatively regulate the length of primary cilia via actin remodeling; Authors - Tomohiko Iwano ; KD Validated
mouse	WB	Int J Mol Sci; The Cleavage-Specific Tau 12A12mAb Exerts an Anti-Amyloidogenic Action by Modulating the Endocytic and Bioenergetic Pathways in Alzheimer's Disease Mouse Model; Authors - Valentina Latina
mouse	WB,IF	J Am Soc Nephrol; Glomerulosclerosis Induced by Deficiency of Membrane-Associated Guanylate Kinase Inverted 2 in Kidney Podocytes; Authors - Naritoshi Shirata
human	WB	Int J Cardiol; Human cytomegalovirus pUL135 protein affects endothelial cell function via CD2AP in Kawasaki disease; Authors - Lili Yu ; KD Validated

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