Anticorps Polyclonal de lapin anti-Cd68

Cd68 Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

souris et plus (3)

Applications

WB, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

173

Avis (2)

N° de cat : 28058-1-AP

Synonymes

Cd68, CD68 antigen, gp110, Scard1

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Galerie de données de validation

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  WB analysis of RAW 264.7 using 28058-1-AP

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 28058-1-AP (Cd68 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse spleen using 28058-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 28058-1-AP (Cd68 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse spleen using 28058-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 28058-1-AP (Cd68 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse spleen using 28058-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 28058-1-AP (Cd68 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 28058-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 28058-1-AP (Cd68 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 28058-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 28058-1-AP (Cd68 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse spleen using 28058-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse spleen tissue using Cd68 antibody (28058-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse spleen using 28058-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse spleen tissue using Cd68 antibody (28058-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of RAW 264.7 using 28058-1-AP

  1X10^6 RAW 264.7 cells were intracellularly stained with 0.4 ug Anti-Mouse Cd68 (28058-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules RAW 264.7, tissu splénique de souris
• Résultats positifs en IHC: tissu splénique de souris, tissu hépatique de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: tissu splénique de souris,
• Résultats positifs en cytométrie: cellules RAW 264.7,

Dilution recommandée

• Western Blot (WB): WB : 1:600-1:2000
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 35 publications below
• IHC: See 75 publications below
• IF: See 70 publications below
• FC: See 2 publications below

Informations sur le produit

28058-1-AP cible Cd68 dans les applications de WB, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Réactivité citée: rat, canin, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Cd68 Protéine recombinante Ag27827
• Nom complet: CD68 antigen
• Masse moléculaire calculée: 35 kDa
• Poids moléculaire observé: 100 kDa
• Numéro d’acquisition GenBank: NM_001291058
• Symbole du gène: Cd68
• Identification du gène (NCBI): 12514
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Mouse CD68 (also known as macrosialin) is a type I transmembrane glycoprotein that is highly and specifically expressed by mouse tissue macrophages, and to a lesser extent by dendritic cells. It belongs to the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family and primarily localizes to lysosomes and endosomes with a smaller fraction circulating to the cell surface. CD68 is also a member of the scavenger receptor family. It may play a role in phagocytic activities of tissue macrophages.

Publications

Species	Application	Title
mouse	IHC	Crit Care; Recombinant ACE2 protein protects against acute lung injury induced by SARS-CoV-2 spike RBD protein.; Authors - Lingbing Zhang
mouse	IF	J Pineal Res; Extracellular vesicles derived from melatonin-preconditioned mesenchymal stem cells containing USP29 repair traumatic spinal cord injury by stabilizing NRF2.; Authors - Wei Liu
mouse	IF	J Clin Invest; Circular RNA circEsyt2 regulates vascular smooth muscle cell remodeling via splicing regulation.; Authors - Xue Gong
	IF	J Exp Clin Cancer Res; FAM171B stabilizes vimentin and enhances CCL2-mediated TAM infiltration to promote bladder cancer progression; Authors - Wei-Min Hu
mouse	IF	Acta Biomater; Porous composite hydrogels with improved MSC survival for robust epithelial sealing around implants and M2 macrophage polarization; Authors - Yongzheng Li
mouse	IHC	Biomaterials; Dual targeted delivery of statins and nucleic acids by chitosan-based nanoparticles for enhanced antiatherosclerotic efficacy.; Authors - Ting Jiang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Alexandru (Verified Customer) (11-13-2023)

Great antibody, worked well in my experiments.

• Applications: Immunofluorescence
• Cell Tissue Type: Mouse ventricle

FH

Amelie (Verified Customer) (09-09-2019)

sections were deparaffinized and subjected to heat mediated antigen retrieval using TE buffer ph9On the pictures CD68 in red

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: mouse adrenal gland

Cd68 Antibody Immunofluorescence, validation (1:1000 dilution) in mouse adrenal gland (Cat no:28058-1-AP)