Anticorps Recombinant de lapin anti-Chemerin

Chemerin Recombinant Antibody for WB, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

souris

Applications

WB, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

240355A12

N° de cat : 83550-4-RR

Synonymes

Rarres2, Retinoic acid receptor responder protein 2, AI303516, 240355A12

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Galerie de données de validation

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  WB analysis using 83550-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83550-4-RR (Chemerin antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  FC experiment of C57BL/6 mouse liver cells using 83550-4-RR

  1x10^6 C57BL/6 mouse liver cells were intracellularly stained with 0.25 ug Chemerin Recombinant antibody (83550-4-RR, Clone:240355A12) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 83550-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83550-4-RR against Mouse Chemerin were performed. The affinity constant is 3.76 nM.

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Applications testées

• Résultats positifs en WB: tissu adipeux de souris, tissu adipeux brun de souris
• Résultats positifs en FC (Intra): C57BL/6 mouse liver cells,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:16000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

83550-4-RR cible Chemerin dans les applications de WB, FC (Intra), ELISA et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: retinoic acid receptor responder (tazarotene induced) 2
• Masse moléculaire calculée: 18 kDa
• Poids moléculaire observé: 14-19 kDa
• Numéro d’acquisition GenBank: NM_001347168.1
• Symbole du gène: Chemerin
• Identification du gène (NCBI): 71660
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Chemerin, also named as TIG2 and RARRES2, is adipocyte-secreted protein (adipokine) that regulates adipogenesis, metabolism and inflammation through activation of the chemokine-like receptor 1 (CMKLR1). Its other ligands include G protein-coupled receptor 1 (GPR1) and chemokine receptor-like 2 (CCRL2). It positively regulates adipocyte differentiation, modulates the expression of adipocyte genes involved in lipid and glucose metabolism and might play a role in angiogenesis, a process essential for the expansion of white adipose tissue. RARRES2 have both pro- and anti-inflammatory properties depending on the modality of enzymatic cleavage by different classes of proteases. The MW of Chemerin is 14-19 kDa.

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