Anticorps Monoclonal anti-Chromogranin A

Chromogranin A Monoclonal Antibody for FC (Intra), IF

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat, souris

Applications

IF, FC (Intra)

Conjugaison

CoraLite®555 Fluorescent Dye

CloneNo.

3H11C11

N° de cat : CL555-60135

Synonymes

CGA, CHGA, Chromogranin A, Pituitary secretory protein I, SP I, Vasostatin I Vasostatin 2

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Galerie de données de validation

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  IF Staining of PC-12 using CL555-60135

  Immunofluorescent analysis of (-20°C Ethanol) fixed PC-12 cells using CoraLite®555 Chromogranin A antibody (CL555-60135, Clone: 3H11C11 ) at dilution of 1:200.

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  IF Staining of SH-SY5Y using CL555-60135

  Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using CoraLite®555 Chromogranin A antibody (CL555-60135, Clone: 3H11C11 ) at dilution of 1:200.

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  IF Staining of Neuro-2a using CL555-60135

  Immunofluorescent analysis of (4% PFA) fixed Neuro-2a cells using CoraLite®555 Chromogranin A antibody (CL555-60135, Clone: 3H11C11 ) at dilution of 1:200.

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  FC experiment of SH-SY5Y using CL555-60135

  1X10^6 SH-SY5Y cells were intracellularly stained with 0.8 ug CoraLite®555 Anti-Human Chromogranin A (CL555-60135, Clone:3H11C11) (red), or 0.8 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  FC experiment of Neuro-2a using CL555-60135

  1X10^6 Neuro-2a cells were intracellularly stained with 0.8 ug CoraLite®555 Anti-Human Chromogranin A (CL555-60135, Clone:3H11C11) (red), or 0.8 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en IF: cellules PC-12, cellules Neuro-2a, cellules SH-SY5Y
• Résultats positifs en cytométrie: cellules SH-SY5Y, cellules Neuro-2a

Dilution recommandée

• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

CL555-60135 cible Chromogranin A dans les applications de IF, FC (Intra) et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Chromogranin A Protéine recombinante Ag0807
• Nom complet: chromogranin A (parathyroid secretory protein 1)
• Masse moléculaire calculée: 51 kDa
• Numéro d’acquisition GenBank: BC006459
• Symbole du gène: CHGA
• Identification du gène (NCBI): 1113
• Conjugaison: CoraLite®555 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 557 nm / 570 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec glycérol à 50 %, Proclin300 à 0,05 % et BSA à 0,5 %, pH 7,3.
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Chromogranin A is a member of the granin family of neuroendocrine secretory proteins. It is located in secretory vesicles of neurons and endocrine cells. Chromogranin A is the precursor to several functional peptides including vasostatin, pancreastatin, catestatin and parastatin. These peptides negatively modulate the neuroendocrine function of the releasing cell (autocrine) or nearby cells (paracrine). CgA is one of the most used tumor markers in NET's (neuroendocrine tumors) , and elevated CgA concentrations have been demonstrated in serum or plasma of patients with different types of these tumors.