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Anticorps Monoclonal anti-Chromogranin A

Chromogranin A Monoclonal Antibody for FC (Intra)

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat, souris

Applications

FC (Intra)

Conjugaison

CoraLite®594 Fluorescent Dye

CloneNo.

3H11C11

N° de cat : CL594-60135

Synonymes

CGA, CHGA, Chromogranin A, Pituitary secretory protein I, SP I, Vasostatin I Vasostatin 2

Galerie de données de validation

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  • Flow cytometry (FC) experiment of SH-SY5Y cells using CoraLite®594-conjugated Chromogranin A Monoclonal (CL594-60135)

    FC experiment of SH-SY5Y using CL594-60135

    1X10^6 SH-SY5Y cells were intracellularly stained with 0.8 ug CoraLite®594 Anti-Human Chromogranin A (CL594-60135, Clone:3H11C11) (red), or 0.8 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Flow cytometry (FC) experiment of Neuro-2a cells using CoraLite®594-conjugated Chromogranin A Monoclonal (CL594-60135)

    FC experiment of Neuro-2a using CL594-60135

    1X10^6 Neuro-2a cells were intracellularly stained with 0.8 ug CoraLite®594 Anti-Human Chromogranin A (CL594-60135, Clone:3H11C11) (red), or 0.8 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Applications testées

Résultats positifs en cytométriecellules SH-SY5Y, cellules Neuro-2a

Dilution recommandée

ApplicationDilution
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

CL594-60135 cible Chromogranin A dans les applications de FC (Intra) et montre une réactivité avec des échantillons Humain, porc, rat, souris

Réactivité Humain, porc, rat, souris
Hôte / Isotype Mouse / IgG1
Clonalité Monoclonal
Type Anticorps
Immunogène Chromogranin A Protéine recombinante Ag0807
Nom complet chromogranin A (parathyroid secretory protein 1)
Masse moléculaire calculée 51 kDa
Poids moléculaire observé 70 kDa
Numéro d’acquisition GenBankBC006459
Symbole du gène CHGA
Identification du gène (NCBI) 1113
Conjugaison CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Forme Liquide
Méthode de purification Purification par protéine G
Tampon de stockage PBS avec glycérol à 50 %, Proclin300 à 0,05 % et BSA à 0,5 %, pH 7,3.
Conditions de stockageStocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Chromogranin A is a member of the granin family of neuroendocrine secretory proteins. It is located in secretory vesicles of neurons and endocrine cells. Chromogranin A is the precursor to several functional peptides including vasostatin, pancreastatin, catestatin and parastatin. These peptides negatively modulate the neuroendocrine function of the releasing cell (autocrine) or nearby cells (paracrine). CgA is one of the most used tumor markers in NET's (neuroendocrine tumors) , and elevated CgA concentrations have been demonstrated in serum or plasma of patients with different types of these tumors.

Protocole

Product Specific Protocols
FC protocol for CL594 Chromogranin A antibody CL594-60135Download protocol
Standard Protocols
Click here to view our Standard Protocols