Anticorps Polyclonal de lapin anti-Claudin 2

Claudin 2 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (3)

Applications

WB, IF, ELISA

Conjugaison

Non conjugué

Publications(15)

N° de cat : 26912-1-AP

Synonymes

CLDN2, SP82, PSEC0059

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Galerie de données de validation

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  WB analysis using 26912-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26912-1-AP (Claudin 2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 26912-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26912-1-AP (Claudin 2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules A549, cellules Caco-2, cellules HEK-293, cellules HepG2, cellules HT-29

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 10 publications below
• IF: See 6 publications below

Informations sur le produit

26912-1-AP cible Claudin 2 dans les applications de WB, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, Humain, poulet, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Claudin 2 Protéine recombinante Ag25527
• Nom complet: claudin 2
• Masse moléculaire calculée: 25 kDa
• Poids moléculaire observé: 25 kDa
• Numéro d’acquisition GenBank: BC014424
• Symbole du gène: Claudin 2
• Identification du gène (NCBI): 9075
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Claudin 2, also named as SP82, belongs to the claudin family. Claudin 2 is a tetraspan membrane protein consisting of two extracellular domains (ECL1 and ECL2), a small intracellular loop connecting the second and third transmembrane sections and short intracellular N and C terminal portions. Claudin 2 is a cation-selective channel forming TJ protein expressed in leaky epithelia. Claudin 2 is a 230 amino acid transmembrane protein, with a calculated molecular mass of 25 kDa (PMID: 31726679)

Publications

Species	Application	Title
mouse	IF	J Ethnopharmacol; The rhizomes of Atractylodes macrocephala Koidz improve gastrointestinal health and pregnancy outcomes in pregnant mice via modulating intestinal barrier and water-fluid metabolism; Authors - Wang Chenxing
human	WB	Bioconjug Chem; Transforms of Cell Surface Glycoproteins Charge Influences Tumor Cell Metastasis via Atypically Inhibiting Epithelial-Mesenchymal Transition Including Matrix Metalloproteinases and Cell Junctions; Authors - Mingzhe Wang
rat	WB	Pharmaceuticals (Basel); Exploring the Underlying Mechanism of Weiling Decoction Alleviates Cold-Dampness Diarrhea Based on Network Pharmacology, Transcriptomics, Molecular Docking and Experimental Validation; Authors - Yannan Zhang
mouse	WB	Naunyn Schmiedebergs Arch Pharmacol; 5-aminosalicylic acid alleviates colitis and protects intestinal barrier function by modulating gut microbiota in mice; Authors - Jingjing Wang
mouse	IF	Front Nutr; Lactobacillus fermentum CECT5716 Alleviates the Inflammatory Response in Asthma by Regulating TLR2/TLR4 Expression; Authors - Weifang Wang
mouse	IF	Diabetes Metab Syndr Obes; Effects of Different Carbohydrate Content Diet on Gut Microbiota and Aortic Calcification in Diabetic Mice; Authors - Xinyi Shen

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