Anticorps Monoclonal anti-Collagen Type I

Collagen Type I Monoclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc et plus (1)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

123

CloneNo.

1E9A7

Avis (6)

N° de cat : 67288-1-Ig

Synonymes

Alpha 1 type I collagen, COL1A1, Collagen alpha 1(I) chain, collagen, type I, alpha 1, OI4

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Galerie de données de validation

• 

  WB analysis of pig colon using 67288-1-Ig

  pig colon tissue were subjected to SDS PAGE followed by western blot with 67288-1-Ig (Collagen Type I antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 67288-1-Ig

  human placenta tissue were subjected to SDS PAGE followed by western blot with 67288-1-Ig (Collagen Type I antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human cervical cancer using 67288-1-Ig

  human cervical cancer tissue were subjected to SDS PAGE followed by western blot with 67288-1-Ig (Collagen Type I antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig lung using 67288-1-Ig

  pig lung tissue were subjected to SDS PAGE followed by western blot with 67288-1-Ig (Collagen Type I antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig skin using 67288-1-Ig

  pig skin tissue were subjected to SDS PAGE followed by western blot with 67288-1-Ig (Collagen Type I antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 67288-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67288-1-Ig (Collagen Type I antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 67288-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67288-1-Ig (Collagen Type I antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 67288-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67288-1-Ig (Collagen Type I antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 67288-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67288-1-Ig (Collagen Type I antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 67288-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 67288-1-Ig (Collagen Type I antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 67288-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 67288-1-Ig (Collagen Type I antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human colon cancer using 67288-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using Collagen Type I antibody (67288-1-Ig, Clone: 1E9A7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of human colon cancer using 67288-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using Collagen Type I antibody (67288-1-Ig, Clone: 1E9A7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu de côlon de porc, tissu cutané de porc, tissu de cancer du col de l'utérus humain, tissu placentaire humain, tissu pulmonaire de porc
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu de cancer du côlon humain, tissu de côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: tissu de cancer du côlon humain,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:2500-1:10000
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 95 publications below
• IHC: See 26 publications below
• IF: See 25 publications below

Informations sur le produit

67288-1-Ig cible Collagen Type I dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, porc

• Réactivité: Humain, porc
• Réactivité citée: Humain, Lapin
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: collagen, type I, alpha 1
• Masse moléculaire calculée: 139 kDa
• Poids moléculaire observé: 120-130 kDa
• Numéro d’acquisition GenBank: NM_000088
• Symbole du gène: COL1A1
• Identification du gène (NCBI): 1277
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Type I collagen, the major structural component of connective tissues such as skin, tendon and bone, is the most abundant and widely expressed collagen in humans (PMID: 7620364; 8645190; 9016532). Type I collagen is a heterotrimer comprising one alpha 2(I) and two alpha 1(I) chains which are encoded by the unlinked loci COL1A2 and COL1A1 respectively. Mutations in COL1A1 are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIA, Ehlers-Danlos syndrome Classical type, Caffey Disease and idiopathic osteoporosis. This antibody raised against a synthesized peptide corresponding to 1206-1218 aa of human pro-alpha 1 chain of type I collagen recognize collagen alpha-1(I) chain. The presence of unprocessed, intermediate, and mature chains of type I collagen was clearly detected only in static constructs. Indeed, in sponges cultured under perfusion the presence of type I collagen was mainly restricted to mature chains, suggesting that HACs were no longer actively producing type I collagen (PMID: 27584727).

Publications

Species	Application	Title
human	IHC	J Hematol Oncol; Proteogenomic insights into the biology and treatment of pancreatic ductal adenocarcinoma; Authors - Yexin Tong
human	IF	Nat Cell Biol; Persistence of a regeneration-associated, transitional alveolar epithelial cell state in pulmonary fibrosis.; Authors - Yoshihiko Kobayashi
human	IHC	Adv Sci (Weinh); Silencing of NOTCH3 Signaling in Meniscus Smooth Muscle Cells Inhibits Fibrosis and Exacerbates Degeneration in a HEYL-Dependent Manner; Authors - Hao Sun
	WB,IHC	Small; M2 Macrophage Membrane-Mediated Biomimetic-Nanoparticle Carrying COX-siRNA Targeted Delivery for Prevention of Tendon Adhesions by Inhibiting Inflammation; Authors - Jie Sun
	WB	Eur Respir J; Intracellular hydroxyproline imprinting following resolution of bleomycin-induced pulmonary fibrosis.; Authors - Shengren Song
	WB	Nat Commun; Tartrate-resistant acid phosphatase 5 promotes pulmonary fibrosis by modulating β-catenin signaling.; Authors - Yinan Hu

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Niky (Verified Customer) (02-26-2024)

Arrived very quickly on ice. However, didnt work for my tissue type

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:400
• Cell Tissue Type: endometrial lesion

FH

Udesh (Verified Customer) (12-22-2023)

Worked well for IF at 1:400

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:400
• Cell Tissue Type: BM-MSCs

FH

Niklas (Verified Customer) (11-08-2022)

great staining with even a high dilution factor

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:400
• Cell Tissue Type: human fibroblasts

FH

Ren Jie (Verified Customer) (06-22-2022)

Does not work for pepsin digested human collagen I

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Purified protein

FH

Hala (Verified Customer) (02-26-2021)

very good

• Applications: Western Blot
• Primary Antibody Dilution: 1/5000

FH

Hala (Verified Customer) (02-23-2021)

very good

• Applications: Western Blot
• Primary Antibody Dilution: 1/5000