Anticorps Monoclonal anti-Collagen Type I

Collagen Type I Monoclonal Antibody for IF-P

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, souris

Applications

IF-P

Conjugaison

CoraLite®594 Fluorescent Dye

Publications(1)

CloneNo.

1E9A7

Avis (1)

N° de cat : CL594-67288

Synonymes

COL1A1, col1, COL I, Alpha-1 type I collagen, Alpha 1 type I collagen

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Galerie de données de validation

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  IF Staining of human colon cancer using CL594-67288

  Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using CoraLite®594 Collagen Type I antibody (CL594-67288, Clone: 1E9A7 ) at dilution of 1:200.

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  IF Staining of human colon cancer using CL594-67288

  Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using CoraLite®594 Collagen Type I antibody (CL594-67288, Clone: 1E9A7 ) at dilution of 1:200.

• 

  IF Staining of mouse skin using CL594-67288

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse skin tissue using CoraLite®594 Collagen Type I antibody (CL594-67288, Clone: 1E9A7 ) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human breast cancer using CL594-67288

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using CoraLite®594 Collagen Type I antibody (CL594-67288, Clone: 1E9A7 ) at dilution of 1:200.

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Applications testées

• Résultats positifs en IF-P: tissu de cancer du côlon humain, tissu cutané de souris, tissu de cancer du sein humain

Dilution recommandée

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IF: See 1 publications below

Informations sur le produit

CL594-67288 cible Collagen Type I dans les applications de IF-P et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: collagen, type I, alpha 1
• Masse moléculaire calculée: 139 kDa
• Poids moléculaire observé: 120-130 kDa
• Numéro d’acquisition GenBank: NM_000088
• Symbole du gène: COL1A1
• Identification du gène (NCBI): 1277
• Conjugaison: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Type I collagen, the major structural component of connective tissues such as skin, tendon and bone, is the most abundant and widely expressed collagen in humans (PMID: 7620364; 8645190; 9016532). Type I collagen is a heterotrimer comprising one alpha 2(I) and two alpha 1(I) chains which are encoded by the unlinked loci COL1A2 and COL1A1 respectively. Mutations in COL1A1 are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIA, Ehlers-Danlos syndrome Classical type, Caffey Disease and idiopathic osteoporosis. This antibody raised against a synthesized peptide corresponding to 1206-1218 aa of human pro-alpha 1 chain of type I collagen recognize collagen alpha-1(I) chain.

Publications

Species	Application	Title
human	IF	Int J Biol Sci; Bulk and single-cell transcriptome profiling reveal extracellular matrix mechanical regulation of lipid metabolism reprograming through YAP/TEAD4/ACADL axis in hepatocellular carcinoma; Authors - Jingwei Cai

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Areli (Verified Customer) (03-14-2023)

I would use a higher dilution next time as the signal was high.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Y201-MSC cells

CL594 Collagen Type I Antibody Immunofluorescence validation (1:500 dilution) in Y201-MSC cells (Cat no:CL594-67288)

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