Anticorps Polyclonal de lapin anti-Collagen Type II

Collagen Type II Polyclonal Antibody for IHC, IF/ICC, IF-P, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (4)

Applications

WB, IHC, IF/ICC, IF-P, CoIP, ELISA

Conjugaison

Non conjugué

Publications(363)

Avis (2)

N° de cat : 28459-1-AP

Synonymes

COL2, COL2A1, Alpha 1 type II collagen, Alpha-1 type II collagen, ANFH

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Galerie de données de validation

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  IHC staining of human lung using 28459-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 28459-1-AP (Collagen Type II antibody) at dilution of 1:1600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung using 28459-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 28459-1-AP (Collagen Type II antibody) at dilution of 1:1600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human lung cancer using 28459-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using Collagen Type II antibody (28459-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of human lung cancer using 28459-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using Collagen Type II antibody (28459-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of ATDC-5 using 28459-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed ATDC-5 cells using Collagen Type II antibody (28459-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Applications testées

• Résultats positifs en IHC: tissu pulmonaire humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu de cancer du poumon humain,
• Résultats positifs en IF/ICC: cellules ATDC-5,

Dilution recommandée

• Immunohistochimie (IHC): IHC : 1:800-1:3200
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 218 publications below
• IHC: See 142 publications below
• IF: See 150 publications below
• CoIP: See 2 publications below

Informations sur le produit

28459-1-AP cible Collagen Type II dans les applications de WB, IHC, IF/ICC, IF-P, CoIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, Humain, Lapin, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Collagen Type II Protéine recombinante Ag29265
• Nom complet: collagen, type II, alpha 1
• Masse moléculaire calculée: 134 kDa
• Numéro d’acquisition GenBank: BC007252
• Symbole du gène: COL2A1
• Identification du gène (NCBI): 1280
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The COL2A1 gene encodes the alpha-1 chain of type II collagen, a fibrillar collagen found primarily in cartilage and the vitreous humor of the eye. Type II collagen is essential for the formation of the extracellular matrix in cartilage and other connective tissues, providing structure and strength to these tissues .

Publications

Species	Application	Title
mouse,human	IHC,IF	Nature; A plant-derived natural photosynthetic system for improving cell anabolism; Authors - Pengfei Chen
rat	IF	Adv Mater; Injectable Microgels with Hybrid Exosomes of Chondrocyte-Targeted FGF18 Gene-Editing and Self-Renewable Lubrication for Osteoarthritis Therapy; Authors - Manyu Chen
rat	IHC	Bioact Mater; Engineering a stem cell-embedded bilayer hydrogel with biomimetic collagen mineralization for tendon-bone interface healing; Authors - Tingyun Lei
mouse,rat	IHC,IF	Bioact Mater; Synergizing adaptive immunity and regenerative signals to enhance osteochondral defects repair; Authors - Changjian Lin
rat	IF	Nat Commun; Injectable ECM-mimetic dynamic hydrogels abolish ferroptosis-induced post-discectomy herniation through delivering nucleus pulposus progenitor cell-derived exosomes; Authors - Wenkai Wang
human,rat	WB	Adv Sci (Weinh); Mechanical Load-Induced Upregulation of Talin2 through Non-Canonical Deubiquitination of OTUB1 Drives Facet Joint Osteoarthritis Pathogenesis; Authors - Yizhen Huang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Steve (Verified Customer) (07-23-2025)

I used that antibody in flow to detect cells from canine species, so far so good. I am validating at this moment in Western Blot.

• Applications: Western Blot, Flow Cytometry
• Cell Tissue Type: Stem Cell

FH

Balawant (Verified Customer) (12-20-2022)

Ive used this Antibody. this great antibody for IHC.

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: colon Tissue

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