Anticorps Polyclonal de lapin anti-Connexin-26

Connexin-26 Polyclonal Antibody for WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IF, ELISA

Conjugaison

Non conjugué

Publications

1

N° de cat : 14842-1-AP

Synonymes

Connexin 26, Connexin-26, CX26, DFNA3, DFNA3A, DFNB1, DFNB1A, Gap junction beta 2 protein, GJB2, HID, KID, NSRD1, PPK

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Galerie de données de validation

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  WB analysis of human heart using 14842-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 14842-1-AP (Connexin-26 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 14842-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14842-1-AP (Connexin-26 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: tissu cardiaque humain, tissu cérébral de souris

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

14842-1-AP cible Connexin-26 dans les applications de WB, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: gap junction protein, beta 2, 26kDa
• Masse moléculaire calculée: 26 kDa
• Poids moléculaire observé: 26 kDa
• Numéro d’acquisition GenBank: NM_004004
• Symbole du gène: GJB2
• Identification du gène (NCBI): 2706
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The gap junctions were first characterized by electron microscopy as regionally specialized structures on plasma membranes of contacting adherent cells. These structures were shown to consist of cell-to-cell channels that facilitate the transfer of ions and small molecules between cells. The gap junction proteins, also known as connexins, purified from fractions of enriched gap junctions from different tissues differ. According to sequence similarities at the nucleotide and amino acid levels, the gap junction proteins are divided into two categories, alpha and beta. Connexin-26 (GJB2) is a beta class gap junction protein. Mutations in the gene for connexin-26 are responsible for as much as 50% of pre-lingual, recessive deafness.

Publications

Species	Application	Title
human	WB,IF	Elife; Solute exchange through gap junctions lessens the adverse effects of inactivating mutations in metabolite-handling genes; Authors - Stefania Monterisi ; KO Validated