Anticorps Polyclonal de lapin anti-Connexin 43

Connexin 43 Polyclonal Antibody for WB, IHC, IF-P, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF-P, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications(81)

Avis (2)

N° de cat : 26980-1-AP

Synonymes

GJA1, Connexin-43, GJAL, Gap junction alpha-1 protein, Gap junction alpha 1 protein

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Galerie de données de validation

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  WB analysis using 26980-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26980-1-AP (Connexin 43 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 26980-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 26980-1-AP (Connexin 43 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse heart using 26980-1-AP

  IP result of anti-Connexin 43 (IP:26980-1-AP, 4ug; Detection:26980-1-AP 1:500) with mouse heart tissue lysate 4000 ug.

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  IHC staining of rat heart using 26980-1-AP

  Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 26980-1-AP (Connexin 43 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat heart using 26980-1-AP

  Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 26980-1-AP (Connexin 43 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 26980-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 26980-1-AP (Connexin 43 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human heart using 26980-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 26980-1-AP (Connexin 43 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human heart using 26980-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 26980-1-AP (Connexin 43 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of mouse heart using 26980-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using 26980-1-AP (Connexin 43 antibody) at dilution of 1:50 and 66895-1-Ig (alpha Actinin mouse mAb) at dilution of 1:50, further stained with CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) for 26980-1-AP, and CoraLite594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) for 66895-1-Ig.

• 

  IF Staining of mouse heart using 26980-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse heart tissue using Connexin 43 antibody (26980-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse heart using 26980-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse heart tissue using Connexin 43 antibody (26980-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human heart using 26980-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human heart tissue using 26980-1-AP (Connexin 43 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, tissu cardiaque de rat, tissu cardiaque de souris, tissu cérébral de rat, tissu testiculaire de souris
• Résultats positifs en IP: tissu cardiaque de souris,
• Résultats positifs en IHC: tissu cardiaque de rat, tissu cardiaque de souris, tissu cardiaque humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu cardiaque de souris, tissu cardiaque humain

Dilution recommandée

• Western Blot (WB): WB : 1:3000-1:12000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 64 publications below
• IHC: See 11 publications below
• IF: See 29 publications below
• IP: See 3 publications below
• FC: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

26980-1-AP cible Connexin 43 dans les applications de WB, IHC, IF-P, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Connexin 43 Protéine recombinante Ag25533
• Nom complet: gap junction protein, alpha 1, 43kDa
• Masse moléculaire calculée: 43 kDa
• Poids moléculaire observé: 43-47 kDa
• Numéro d’acquisition GenBank: BC026329
• Symbole du gène: Connexin-43
• Identification du gène (NCBI): 2697
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Connexin-43 (Cx43, also known as gap junction alpha-1), a member of connexin family, plays essential roles in gap junction communication that facilitates direct communication among adjacent cells (PMID: 12270943). Usually, six connexin proteins oligomerize into a hemi-channel or connexon during intercellular channel formation (PMID: 12270943). Mutations of Cx43 may cause a series of diseases such as oculodentodigital dysplasia (ODDD), syndactyly 3 (SDTY3), hypoplastic left heart syndrome 1 (HLHS1) and so on (PMID: 18161618, 1472936, 1147490).

Publications

Species	Application	Title
mouse	WB	Acta Pharm Sin B; Converting bacteria into autologous tumor vaccine via surface biomineralization of calcium carbonate for enhanced immunotherapy; Authors - Lina Guo
rat	WB,IF	Adv Healthc Mater; A ROS-Responsive Liposomal Composite Hydrogel Integrating Improved Mitochondrial Function and Pro-Angiogenesis for Efficient Treatment of Myocardial Infarction.; Authors - Zhi Zheng
mouse	WB,IF	Drug Des Devel Ther; Shensong Yangxin Capsule Reduces the Susceptibility of Arrhythmia in db/db Mice via Inhibiting the Inflammatory Response Induced by Endothelium Dysfunction; Authors - Jiehan Zhang
human,mouse	WB	Cell Commun Signal; Extracellular vesicular delivery of ceramides from pulmonary macrophages to endothelial cells facilitates chronic obstructive pulmonary disease; Authors - Qiqing Huang
mouse	WB,IF	Cell Mol Biol Lett; Robo4 inhibits gamma radiation-induced permeability of a murine microvascular endothelial cell by regulating the junctions; Authors - Seyram Yao Adzraku
mouse	WB	Cell Mol Life Sci; Connexin 43 contributes to perioperative neurocognitive disorder by attenuating perineuronal net of hippocampus in aged mice; Authors - Qian Zhang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Muhammad (Verified Customer) (08-30-2022)

Cx43 antibody used is excellent and working in HUVEC cells.

• Applications: Western Blot
• Cell Tissue Type: HUVEC

FH

ABDULLAH (Verified Customer) (11-08-2019)

This antibody worked great!It detected the protein at the right size.There wasn't any background.

• Applications: Western Blot, Immunofluorescence,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: BEND.3 cells

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