Anticorps Monoclonal anti-Cyclin A2

• Phare
• Validé par KD/KO

Cyclin A2 Monoclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain et plus (2)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

22

CloneNo.

1C6C11

Avis (1)

N° de cat : 66391-1-Ig

Synonymes

CCN1, CCNA, CCNA2, Cyclin A, Cyclin A2, cyclinA2

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Galerie de données de validation

• 

  WB analysis using 66391-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66391-1-Ig (Cyclin A2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 66391-1-Ig

  WB result of Cyclin A2 antibody (66391-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Cyclin A2 transfected HeLa cells.

• 

  IHC staining of human tonsillitis using 66391-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66391-1-Ig (Cyclin A2 antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 66391-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66391-1-Ig (Cyclin A2 antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 66391-1-Ig

  Immunofluorescent analysis of (4% PFA ) fixed HeLa cells using 66391-1-Ig(Cyclin A2 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of MCF-7 using 66391-1-Ig

  Immunofluorescent analysis of (4% PFA ) fixed MCF-7 cells using 66391-1-Ig(Cyclin A2 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules HEK-293, cellules HeLa, cellules Jurkat, cellules Raji
• Résultats positifs en IHC: tissu d'amygdalite humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa, cellules MCF-7

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochimie (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 20 publications below
• IF: See 2 publications below

Informations sur le produit

66391-1-Ig cible Cyclin A2 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Cyclin A2 Protéine recombinante Ag12765
• Nom complet: cyclin A2
• Masse moléculaire calculée: 432 aa, 49 kDa
• Poids moléculaire observé: 52 kDa
• Numéro d’acquisition GenBank: BC104783
• Symbole du gène: CCNA2
• Identification du gène (NCBI): 890
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CCNA2 belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. In contrast to cyclin A1, which is present only in germ cells, this cyclin is expressed in all tissues tested. CCNA2 binds and activates CDC2 or CDK2 kinases, and thus promotes both cell cycle G1/S and G2/M transitions.

Publications

Species	Application	Title
human	IF	Dev Cell; Cell cycle regulation of ER membrane biogenesis protects against chromosome missegregation.; Authors - Holly Merta
human	WB	Oncogene; KCTD12 promotes tumorigenesis by facilitating CDC25B/CDK1/Aurora A-dependent G2/M transition.; Authors - Y Zhong
human	IF	Elife; METTL3 promotes homologous recombination repair and modulates chemotherapeutic response in breast cancer by regulating the EGF/RAD51 axis.; Authors - Enjie Li
human	WB	J Cell Mol Med; Cell division cycle associated 8: A novel diagnostic and prognostic biomarker for hepatocellular carcinoma; Authors - Xiao-Han Cui
human	WB	Cell Cycle; Enterovirus 71 mediates cell cycle arrest in S phase through non-structural protein 3D.; Authors - Jinghua Yu
human	WB	Molecules; A Potential Anti-Glioblastoma Compound LH20 Induces Apoptosis and Arrest of Human Glioblastoma Cells via CDK4/6 Inhibition; Authors - Yan Wang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Juri (Verified Customer) (01-20-2024)

Signal present after ICC, but expressing from all the cells.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1 in 100
• Cell Tissue Type: cancer cells