Anticorps Polyclonal de lapin anti-Cyclin D3

• Phare
• Validé par KD/KO

Cyclin D3 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat et plus (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

29

N° de cat : 26755-1-AP

Synonymes

CCND3, cyclin D, Cyclin D3, G1/S specific cyclin D3

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Galerie de données de validation

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  WB analysis of C6 using 26755-1-AP

  C6 cells were subjected to SDS PAGE followed by western blot with 26755-1-AP (Cyclin D3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 26755-1-AP

  WB result of Cyclin D3 antibody (26755-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Cyclin D3 transfected Jurkat cells.

• 

  WB analysis of HeLa using 26755-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 26755-1-AP (Cyclin D3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 26755-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 26755-1-AP (Cyclin D3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 26755-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 26755-1-AP (Cyclin D3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of K-562 using 26755-1-AP

  IP Result of anti-Cyclin D3 (IP:26755-1-AP, 4ug; Detection:26755-1-AP 1:1000) with K-562 cells lysate 2800ug.

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  IHC staining of human pancreas using 26755-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 26755-1-AP (Cyclin D3 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human pancreas using 26755-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 26755-1-AP (Cyclin D3 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of rat kidney using 26755-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 26755-1-AP (Cyclin D3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat kidney using 26755-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 26755-1-AP (Cyclin D3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas using 26755-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 26755-1-AP (Cyclin D3 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human pancreas using 26755-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 26755-1-AP (Cyclin D3 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of HeLa using 26755-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 26755-1-AP (Cyclin D3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules C6, cellules HeLa, cellules Jurkat, cellules K-562
• Résultats positifs en IP: cellules K-562
• Résultats positifs en IHC: tissu pancréatique humain, tissu rénal de rat; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 28 publications below
• IHC: See 3 publications below
• IP: See 1 publications below

Informations sur le produit

26755-1-AP cible Cyclin D3 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Cyclin D3 Protéine recombinante Ag25151
• Nom complet: cyclin D3
• Masse moléculaire calculée: 33 kDa
• Poids moléculaire observé: 33 kDa
• Numéro d’acquisition GenBank: BC011616
• Symbole du gène: CCND3
• Identification du gène (NCBI): 896
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Cyclin D3 belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activtiy is required for cell cycle G1/S transition. This protein has been shown to interact with and be involved in the phosphorylation of tumor suppressor protein Rb. The CDK4 activity associated with this cyclin was reported to be necessary for cell cycle progression through G2 phase into mitosis after UV radiation.

Publications

Species	Application	Title
human	WB,IP	Acta Pharmacol Sin; Inhibition of USP10 induces myeloma cell apoptosis by promoting cyclin D3 degradation; Authors - Yu-Jia Xu ; KD Validated
human	WB	J Proteome Res; Identification of a Proteomic Signature of Senescence in Primary Human Mammary Epithelial Cells; Authors - Alireza Delfarah
human	WB	Transl Lung Cancer Res; Deubiquitinase USP5 promotes non-small cell lung cancer cell proliferation by stabilizing cyclin D1.; Authors - Zhiwei Zhang
human	IHC	Cancer Gene Ther; Targeting LRIG2 overcomes resistance to EGFR inhibitor in glioblastoma by modulating GAS6/AXL/SRC signaling.; Authors - Minhai Dong
mouse	WB	J Cell Mol Med; Cell cycle exit during bortezomib-induced osteogenic differentiation of mesenchymal stem cells was mediated by Xbp1s-upregulated p21Cip1 and p27Kip1.; Authors - Dan Zhang
human	WB	Mol Ther Oncolytics; S119N Mutation of the E3 Ubiquitin Ligase SPOP Suppresses SLC7A1 Degradation to Regulate Hepatoblastoma Progression.; Authors - Weijing He