Anticorps Monoclonal anti-Cytochrome c

• Phare
• Validé par KD/KO

Cytochrome c Monoclonal Antibody for FC, IF, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

92

CloneNo.

2D8D11

Avis (1)

N° de cat : 66264-1-Ig

Synonymes

CYC, CYCS, Cytochrome c, cytochrome c, somatic, HCS, THC4

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Galerie de données de validation

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  WB analysis using 66264-1-Ig

  Various cells were subjected to SDS PAGE followed by western blot with 66264-1-Ig (Cytochrome c antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 66264-1-Ig

  HEK-293 cells and HEK-293-derived exosomes (HEK-293-exo) were subjected to SDS PAGE followed by western blot with 66264-1-Ig (Cytochrome c antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 66264-1-Ig

  WB result of Cytochrome c antibody (66264-1-Ig; 1:200000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Cytochrome c transfected HEK-293 cells.

• 

  WB analysis of human heart using 66264-1-Ig

  human heart tissue were subjected to SDS PAGE followed by western blot with 66264-1-Ig (Cytochrome c Antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human skeletal muscle using 66264-1-Ig

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 66264-1-Ig (Cytochrome c Antibody) at dilution of 1:16000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat skeletal muscle using 66264-1-Ig

  rat skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 66264-1-Ig (Cytochrome c Antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 66264-1-Ig

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 66264-1-Ig (Cytochrome c Antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 66264-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66264-1-Ig (Cytochrome c antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 66264-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66264-1-Ig (Cytochrome c antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 66264-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66264-1-Ig (Cytochrome c antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 66264-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66264-1-Ig (Cytochrome c antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 66264-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66264-1-Ig (Cytochrome c antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 66264-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66264-1-Ig (Cytochrome c antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 66264-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 66264-1-Ig (Cytochrome c antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). Cells were co-stained with phalloidin in red.

• 

  IF Staining of HepG2 using 66264-1-Ig

  Immunofluorescent analysis of (-20℃ Ethanol ) fixed HepG2 cells using 66264-1-Ig(Cytochrome c antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  FC experiment of HepG2 using 66264-1-Ig

  1X10^6 HepG2 cells were intracellularly stained with 0.2 ug Anti-Human Cytochrome c (66264-1-Ig, Clone:2D8D11) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Mouse IgG2a Isotype Control (66360-2-Ig, Clone: K11A1B2A2) (black). Cells were fixed with 4% PFA and permeabilized with 0.1% TritonX-100.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HEK-293, cellules HSC-T6, cellules Jurkat, cellules MCF-7, cellules RAW 264.7, cellules ROS1728, tissu cardiaque humain, tissu de muscle squelettique de rat, tissu de muscle squelettique de souris, tissu de muscle squelettique humain
• Résultats positifs en IHC: tissu de cancer du foie humain, tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2,
• Résultats positifs en cytométrie: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:1000-1:5000
• Immunofluorescence (IF): IF : 1:20-1:200
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 85 publications below
• IF: See 12 publications below

Informations sur le produit

66264-1-Ig cible Cytochrome c dans les applications de WB, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, canin, Humain, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Cytochrome c Protéine recombinante Ag24349
• Nom complet: cytochrome c, somatic
• Masse moléculaire calculée: 12 kDa
• Poids moléculaire observé: 12-15 kDa
• Numéro d’acquisition GenBank: BC009578
• Symbole du gène: CYCS
• Identification du gène (NCBI): 54205
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Cytochrome c is a 12-15 kDa electron transporting protein located in the inner mitochondrial membrane. Upon apoptotic stimulation, cytochrome c can be released from mitochondria into cytoplasm, resulting in caspase-3 activation and apoptosis. Measurement of cytochrome c release from the mitochondria is useful for detection of the onset of apoptosis in cells. In addition, cytochrome c can also leave cells and be detectable in extra-cellular medium of apoptotic cells and serum of cancer patients. The level of serum cytochrome c may serve as a prognostic maker during cancer therapy.

Publications

Species	Application	Title
human,mouse	WB	Hepatology; Hepatic stimulator substance resists hepatic ischemia/reperfusion injury by regulating Drp1 translocation and activation.; Authors - Chao Zhang
human	IF	Nat Commun; Endonuclease G promotes autophagy by suppressing mTOR signaling and activating the DNA damage response.; Authors - Wenjun Wang
human	WB	Redox Biol; Mecheliolide elicits ROS-mediated ERS driven immunogenic cell death in hepatocellular carcinoma.; Authors - Zhongren Xu
human	IF	J. Pineal Res.; Human transporters, PEPT1/2, facilitate melatonin transportation into mitochondria of cancer cells: an implication of the therapeutic potential.; Authors - Xiaokui Huo
human,mouse	WB	Sci China Life Sci; Melatonin decreases GSDME mediated mesothelial cell pyroptosis and prevents peritoneal fibrosis and ultrafiltration failure; Authors - Hongxia Ruan
mouse	WB	Br J Pharmacol; Substitution of SERCA2 Cys674 accelerates aortic aneurysm by inducing endoplasmic reticulum stress and promoting cell apoptosis.; Authors - Langtao Wang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Megha (Verified Customer) (07-10-2018)

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Capan-1 cells

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