Anticorps Recombinant de lapin anti-Cytokeratin 13

Cytokeratin 13 Recombinant Antibody for IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

230366E8

N° de cat : 83058-4-RR

Synonymes

KRT13, 230366E8, CK 13, CK13, CK-13

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Galerie de données de validation

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  IF Staining of A431 using 83058-4-RR

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using Cytokeratin 13 antibody (83058-4-RR, Clone: 230366E8 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  FC experiment of A431 using 83058-4-RR

  1x10^6 A431 cells were intracellularly stained with 0.25 ug Anti-Human KRT13 (83058-4-RR, Clone:230366E8) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 83058-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83058-4-RR against Human Cytokeratin 13 were performed. The affinity constant is 1.65 nM.

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Applications testées

• Résultats positifs en IF/ICC: cellules A431,
• Résultats positifs en FC (Intra): cellules A431

Dilution recommandée

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

83058-4-RR cible Cytokeratin 13 dans les applications de IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Cytokeratin 13 Protéine recombinante Ag0217
• Nom complet: keratin 13
• Masse moléculaire calculée: 50 kDa
• Numéro d’acquisition GenBank: BC002661
• Symbole du gène: Cytokeratin 13
• Identification du gène (NCBI): 3860
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Keratin 13 is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Most of the type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. This type I cytokeratin is paired with keratin 4 and expressed in the suprabasal layers of non-cornified stratified epithelia. Mutations in keratin 13 gene and keratin 4 have been associated with the autosomal dominant disorder White Sponge Nevus. The type I cytokeratins are clustered in a region of chromosome 17q21.2.

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