Anticorps Monoclonal anti-Cytokeratin 18

Cytokeratin 18 Monoclonal Antibody for IF/ICC, FC (Intra)

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat, souris et plus (1)

Applications

IF/ICC, FC (Intra)

Conjugaison

FITC Fluorescent Dye

Publications(4)

CloneNo.

1G11C4

N° de cat : FITC-66187

Synonymes

CK 18, CYK18, Cytokeratin 18, K18, keratin 18, KRT18

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Galerie de données de validation

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  IF Staining of HeLa using FITC-66187

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using FITC Cytokeratin 18 antibody (FITC-66187, Clone: 1G11C4 ) at dilution of 1:200.

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  FC experiment of HeLa using FITC-66187

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug FITC Anti-Human Cytokeratin 18 (FITC-66187, Clone:1G11C4) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en IF/ICC: cellules HeLa,
• Résultats positifs en FC (Intra): cellules HeLa
• Résultats positifs en cytométrie: cellules HeLa

Dilution recommandée

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IF: See 3 publications below
• FC: See 1 publications below

Informations sur le produit

FITC-66187 cible Cytokeratin 18 dans les applications de IF/ICC, FC (Intra) et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Réactivité citée: bovin, Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Cytokeratin 18 Protéine recombinante Ag1260
• Nom complet: keratin 18
• Masse moléculaire calculée: 48 kDa
• Poids moléculaire observé: 48 kDa
• Numéro d’acquisition GenBank: BC000180
• Symbole du gène: Cytokeratin 18
• Identification du gène (NCBI): 3875
• Conjugaison: FITC Fluorescent Dye
• Excitation/Emission maxima wavelengths: 498 nm / 526 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. KRT18, also named as CYK18, PIG46 and K18, is the most commonly found members of the intermediate filament gene family. KRT18 is a type I keratin which is found primarily in non squamous epithelia and is present in a majority of adenocarcinomas and ductal carcinomas but not in squamous cell carcinomas.

Publications

Species	Application	Title
bovine	FC	J Dairy Sci; Role of sortilin 1 (SORT1) on lipid metabolism in bovine liver; Authors - Wei Yang
bovine	IF	J Dairy Sci; Diacylglycerol O-acyltransferase (DGAT) isoforms play a role in peridroplet mitochondrial fatty acid metabolism in bovine liver; Authors - Shuang Wang
human	IF	Exp Ther Med; Detection of PD‑L1 expression and epithelial‑mesenchymal transition of circulating tumor cells in non‑small cell lung cancer; Authors - Jianping Jiang
bovine	IF	J Dairy Sci; Caveolin 1 in bovine liver is associated with fatty acid-induced lipid accumulation and the ER unfolded protein response: role in fatty liver development; Authors - Ming Li

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