Anticorps Polyclonal de lapin anti-Cytokeratin 19

Cytokeratin 19 Polyclonal Antibody for IF/ICC, IF-Fro, FC (Intra)

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

IF/ICC, IF-Fro, FC (Intra)

Conjugaison

CoraLite® Plus 488 Fluorescent Dye

N° de cat : CL488-10712

Synonymes

CK 19, CK19, KRT19, CK-19, Cytokeratin,Keratin

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Galerie de données de validation

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  IF Staining of mouse small intestine using CL488-10712

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse small intestine tissue using CoraLite® Plus 488 Cytokeratin 19 antibody (CL488-10712) at dilution of 1:200.

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  IF Staining of mouse small intestine using CL488-10712

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse small intestine tissue using CoraLite® Plus 488 Cytokeratin 19 antibody (CL488-10712) at dilution of 1:200.

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  IF Staining of HeLa using CL488-10712

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite® Plus 488 Cytokeratin 19 antibody (CL488-10712) at dilution of 1:200.

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  IF Staining of HeLa using CL488-10712

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite® Plus 488 Cytokeratin 19 antibody (CL488-10712) at dilution of 1:200.

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  IF Staining of HepG2 using CL488-10712

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 Cytokeratin 19 antibody (CL488-10712) at dilution of 1:200.

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  IF Staining of mouse small intestine using CL488-10712

  Adult mouse small intestine stained for Cytokeratin 19 (green, CL488-10712) and BMI1 (red, 10832-1-AP). BMI1 stains intestinal stem cells located at the base of the villi. As these stem cells differentiate into intestinal epithelial cells, they acquire Cytokeratin 19 expression. Cytokeratin 19 was conjugated to Coralite 488. Samples were fixed in 4% PFA, embedded in paraffin, and imaged on a confocal microscope. Image credit: @Immunofluorescence on Instagram.

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  FC experiment of MCF-7 using CL488-10712

  1X10^6 MCF-7 cells were intracellularly stained with 0.2 ug CoraLite® Plus 488 Anti-Human Cytokeratin 19 (CL488-10712) (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en IF-Fro: tissu d'intestin grêle de souris,
• Résultats positifs en IF/ICC: cellules HeLa, cellules HepG2
• Résultats positifs en FC (Intra): cellules MCF-7,

Dilution recommandée

• Immunofluorescence (IF)-FRO: IF-FRO : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

CL488-10712 cible Cytokeratin 19 dans les applications de IF/ICC, IF-Fro, FC (Intra) et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Cytokeratin 19 Protéine recombinante Ag1085
• Nom complet: keratin 19
• Masse moléculaire calculée: 40 kDa
• Numéro d’acquisition GenBank: BC007628
• Symbole du gène: Cytokeratin 19
• Identification du gène (NCBI): 3880
• Conjugaison: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells. Keratin expression is highly regulated, tissue specific, and varies according to cell-state. Type I keratins consist of acidic, low molecular weight proteins with MW ranging from 40 kDa (KRT19) to 64 kDa (KRT9). Type 2 keratins consist of basic or neutral, high molecular weight proteins with MW from 52 kDa (KRT8) to 67 kDa (KRT18). Keratin 19 is a type I cytokeratin. It is a biochemical marker of skin stem cells in vivo and in vitro.

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