Anticorps Polyclonal de lapin anti-Cytokeratin 7

Cytokeratin 7 Polyclonal Antibody for IF/ICC, IF-P, FC (Intra)

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

IF/ICC, IF-P, FC (Intra)

Conjugaison

CoraLite®555 Fluorescent Dye

N° de cat : CL555-15539

Synonymes

KRT7, keratin 7, Cytokeratin-7, Cytokeratin,Keratin, CK-7

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Galerie de données de validation

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  IF Staining of human breast cancer using CL555-15539

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using CoraLite®555 Cytokeratin 7 antibody (CL555-15539) at dilution of 1:800, CoraLite® Plus 647 smooth muscle actin specific antibody (CL647-67735, Clone: 1E9A11, Magenta), CoraLite®488 CD31 antibody (CL488-11265, green).

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  IF Staining of human breast cancer using CL555-15539

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using CoraLite®555 Cytokeratin 7 antibody (CL555-15539) at dilution of 1:800, CoraLite® Plus 647 smooth muscle actin specific antibody (CL647-67735, Clone: 1E9A11, Magenta), CoraLite®488 CD31 antibody (CL488-11265, green).

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  IF Staining of HeLa using CL555-15539

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite®555 Cytokeratin 7 antibody (CL555-15539) at dilution of 1:200.

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  IF Staining of HepG2 using CL555-15539

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite®555 Cytokeratin 7 antibody (CL555-15539) at dilution of 1:200.

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  FC experiment of HeLa using CL555-15539

  1x10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite®555-conjugated Cytokeratin 7 Polyclonal antibody (CL555-15539)(red), or 0.8 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en IF-P: tissu de cancer du sein humain
• Résultats positifs en IF/ICC: cellules HeLa, cellules HepG2
• Résultats positifs en FC (Intra): cellules HeLa,

Dilution recommandée

• Immunofluorescence (IF)-P: IF-P : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

CL555-15539 cible Cytokeratin 7 dans les applications de IF/ICC, IF-P, FC (Intra) et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Cytokeratin 7 Protéine recombinante Ag7895
• Nom complet: keratin 7
• Masse moléculaire calculée: 469 aa, 51 kDa
• Poids moléculaire observé: 51 kDa
• Numéro d’acquisition GenBank: BC002700
• Symbole du gène: Cytokeratin 7
• Identification du gène (NCBI): 3855
• Conjugaison: CoraLite®555 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 557 nm / 570 nm
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. KRT7 is a type II keratin. It is specifically expressed in the simple epithelia lining the cavities of the internal organs and in the gland ducts and blood vessels.

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