Anticorps Polyclonal de lapin anti-D2HGDH

• Phare
• Validé par KD/KO

D2HGDH Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

13

N° de cat : 13895-1-AP

Synonymes

D2HGD, D2HGDH

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Galerie de données de validation

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  WB analysis of mouse kidney using 13895-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 13895-1-AP (D2HGDH antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 13895-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 13895-1-AP (D2HGDH antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat liver using 13895-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 13895-1-AP (D2HGDH Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 13895-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 13895-1-AP (D2HGDH antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 13895-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 13895-1-AP (D2HGDH antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 13895-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 13895-1-AP (D2HGDH antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse liver tissue using 13895-1-AP

  IP result of anti-D2HGDH (13895-1-AP for IP and Detection) with mouse liver tissue lysate.

• 

  IP experiment of mouse liver using 13895-1-AP

  IP Result of anti-D2HGDH (IP:13895-1-AP, 3ug; Detection:13895-1-AP 1:500) with mouse liver tissue lysate 5000ug.

• 

  IHC staining of human liver cancer using 13895-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13895-1-AP (D2HGDH Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 13895-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13895-1-AP (D2HGDH Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 13895-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 13895-1-AP (D2HGDH antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu rénal de souris, tissu cardiaque humain, tissu hépatique de rat, tissu hépatique de souris, tissu hépatique humain
• Résultats positifs en IP: tissu hépatique de souris
• Résultats positifs en IHC: tissu de cancer du foie humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 4 publications below
• WB: See 10 publications below
• IHC: See 2 publications below
• IF: See 2 publications below

Informations sur le produit

13895-1-AP cible D2HGDH dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: D2HGDH Protéine recombinante Ag4857
• Nom complet: D-2-hydroxyglutarate dehydrogenase
• Masse moléculaire calculée: 521 aa, 56 kDa
• Poids moléculaire observé: 56 kDa
• Numéro d’acquisition GenBank: BC036604
• Symbole du gène: D2HGDH
• Identification du gène (NCBI): 728294
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

D2HGDH(D-2-hydroxyglutarate dehydrogenase, mitochondrial) is also named as D2HGD and belongs to the FAD-binding oxidoreductase/transferase type 4 family.It catalyzes the oxidation of D-2-hydroxyglutarate to alpha-ketoglutarate.Defects in D2HGDH are the cause of D-2-hydroxyglutaric aciduria type 1 (D2HGA1).It has 2 isoforms produced by alternative splicing.

Publications

Species	Application	Title
human	WB	Cell Metab; Hypoxia Induces Production of L-2-Hydroxyglutarate.; Authors - Andrew M Intlekofer ; KD Validated
human	WB	Cell Metab; Hypoxia-Mediated Increases in L-2-hydroxyglutarate Coordinate the Metabolic Response to Reductive Stress.; Authors - William M Oldham ; KD Validated
human	WB, IF	Nat Commun; D2HGDH regulates alpha-ketoglutarate levels and dioxygenase function by modulating IDH2.; Authors - An-Ping Lin
human	WB	Cell Chem Biol; MYC Regulation of D2HGDH and L2HGDH Influences the Epigenome and Epitranscriptome.; Authors - ZhiJun Qiu ; KO Validated
human	WB	Leukemia; MYC, mitochondrial metabolism and O-GlcNAcylation converge to modulate the activity and subcellular localization of DNA and RNA demethylases.; Authors - An-Ping Lin
	WB	Oncotarget; D-2-hydroxyglutarate is essential for maintaining oncogenic property of mutant IDH-containing cancer cells but dispensable for cell growth.; Authors - Shenghong Ma