Anticorps Monoclonal anti-DACH1

• Phare
• Validé par KD/KO

DACH1 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IF-P, IP, ELISA

Conjugaison

Non conjugué

Publications(4)

CloneNo.

3B6D2

N° de cat : 60082-1-Ig

Synonymes

Dachshund-1, DACH, 3B6D2

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Galerie de données de validation

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  WB analysis of HEK-293 using 60082-1-Ig

  WB result of DACH1 (60082-1-Ig, 1:5000) with si-control and si-DACH1 transfected HEK293 cells.

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  WB analysis of MCF-7 using 60082-1-Ig

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 60082-1-Ig (DACH1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of ROS1728 using 60082-1-Ig

  ROS1728 cells were subjected to SDS PAGE followed by western blot with 60082-1-Ig (DACH1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of C6 using 60082-1-Ig

  C6 cells were subjected to SDS PAGE followed by western blot with 60082-1-Ig (DACH1 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 60082-1-Ig

  L02 cells were subjected to SDS PAGE followed by western blot with 60082-1-Ig (DACH1 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brain using 60082-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 60082-1-Ig (DACH1 antibody) at dilution of 1:1 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human endometrial cancer using 60082-1-Ig

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 60082-1-Ig (DACH1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human endometrial cancer using 60082-1-Ig

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 60082-1-Ig (DACH1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 60082-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 60082-1-Ig (DACH1 antibody) at dilution of 1:1 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 60082-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 60082-1-Ig (DACH1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 60082-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 60082-1-Ig (DACH1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse brain using 60082-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 60082-1-Ig (DACH1 antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of mouse embryo using 60082-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse embryo tissue using DACH1 antibody (60082-1-Ig, Clone: 3B6D2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of mouse embryo using 60082-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse embryo tissue using DACH1 antibody (60082-1-Ig, Clone: 3B6D2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of HEK-293 using 60082-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using DACH1 antibody (60082-1-Ig, Clone: 3B6D2 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules MCF-7, cellules C6, cellules HEK-293, cellules L02, cellules ROS1728
• Résultats positifs en IHC: tissu cérébral de souris, tissu de cancer de la prostate humain, tissu de cancer de l'endomètre humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu embryonnaire de souris, tissu cérébral de souris
• Résultats positifs en IF/ICC: cellules HEK-293

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:10-1:100
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Informations sur le produit

60082-1-Ig cible DACH1 dans les applications de WB, IHC, IF/ICC, IF-P, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: DACH1 Protéine recombinante Ag4474
• Nom complet: dachshund homolog 1 (Drosophila)
• Masse moléculaire calculée: 79 kDa
• Poids moléculaire observé: 97-110 kDa
• Numéro d’acquisition GenBank: BC021219
• Symbole du gène: DACH1
• Identification du gène (NCBI): 1602
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

DACH1, a homologue of the Drosophila dachshund gene, is a key regulator of cell fate determination during eye, leg, and brain development in the fly. Through interacting with NCoR and Smad4, DACH1 is able to inhibit the transforming growth factor-beta (TGF-beta) signaling pathway. DACH1 can inhibit breast cancer cellular proliferation via cyclin D1, suggesting a possible role in tumor suppression. Additionally, DACH1 plays an important role in negative regulation of RANKL (Receptor activator of NF-kappaB ligand ) gene expression in marrow stromal/preosteoblast cells. Dach1 expression is enriched in rECs, which are associated with osteoprogenitors and bone-resorbing osteoclasts, and overexpression of DACH1 in postnatal mice induces a strong increase in arteries and type R capillaries, leading to local metabolic changes and enabling trabecular bone formation in normally highly hypoxic areas of the diaphysis (PMID: 39528700). Moreover, Loss of DACH1 expression might be involved in endometrial cancer progression. Four isoforms of DACH1 are produced by alternative splicing, but isoform1(97-110kd) is the predominantly expressed form in tissue. This antibody is a mouse monoclonal antibody raised against residues near the C terminus of human DACH1.

Publications

Species	Application	Title
human,mouse	WB,IP,IF	Am J Respir Cell Mol Biol; DACH1 Attenuates Airway Inflammation in COPD by Activating NRF2 Signaling; Authors - Qian Huang
human	WB	Cell Biol Int; DACH1 inhibits the proliferation and migration of papillary thyroid carcinoma; Authors - Shengru Liang
human	WB	Front Oncol; Identification and verification of the temozolomide resistance feature gene DACH1 in gliomas; Authors - Qiang Gu ; KD Validated
human	WB	Clin Exp Metastasis; Lung-derived soluble factors support stemness/plasticity and metastatic behaviour of breast cancer cells via the FGF2-DACH1 axis; Authors - Vasudeva Bhat

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