Anticorps Polyclonal de lapin anti-DBH

DBH Polyclonal Antibody for WB, IHC, IF/ICC, IF-Fro, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, IF-Fro, FC (Intra), ELISA

Conjugaison

Non conjugué

Publications(3)

N° de cat : 10777-1-AP

Synonymes

DBM, Dopamine beta-hydroxylase, Dopamine beta-monooxygenase, EC:1.14.17.1, Soluble dopamine beta-hydroxylase

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Galerie de données de validation

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  WB analysis using 10777-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10777-1-AP (DBH antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of SH-SY5Y using 10777-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 10777-1-AP (Dopamine beta Hydroxylase antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 10777-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10777-1-AP (DBH antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 10777-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10777-1-AP (DBH antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 10777-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10777-1-AP (DBH antibody at dilution of 1:100 (under 10x lens).

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  IHC staining of human liver cancer using 10777-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10777-1-AP (DBH antibody at dilution of 1:100 (under 40x lens).

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  IF Staining of rat brain using 10777-1-AP

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded rat brain tissue using DBH antibody (10777-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  IF Staining of SH-SY5Y using 10777-1-AP

  Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using DBH antibody (10777-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  IF Staining of HeLa using 10777-1-AP

  Immunofluorescent analysis of HeLa cells using 10777-1-AP (Dopamine beta Hydroxylase antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of SH-SY5Y using 10777-1-AP

  1X10^6 SH-SY5Y cells were intracellularly stained with 0.4 ug Anti-Human DBH (10777-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules SH-SY5Y, cellules NIH/3T3, tissu cérébral de souris
• Résultats positifs en IHC: tissu cérébral de souris, tissu de cancer du foie humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-Fro: tissu cérébral de rat,
• Résultats positifs en IF/ICC: cellules SH-SY5Y, cellules HeLa
• Résultats positifs en FC (Intra): cellules SH-SY5Y,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-FRO: IF-FRO : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IF: See 1 publications below

Informations sur le produit

10777-1-AP cible DBH dans les applications de WB, IHC, IF/ICC, IF-Fro, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: DBH Protéine recombinante Ag1196
• Nom complet: DBH
• Masse moléculaire calculée: 64.8 kDa
• Poids moléculaire observé: 63 kDa
• Numéro d’acquisition GenBank: BC017174
• Symbole du gène: DBH
• Identification du gène (NCBI): 1621
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

DBH belongs to the copper type II ascorbate-dependent monooxygenase family and catalyzes the oxidative hydroxylation of DBH to norepinephrine. It is almost exclusively located in the adrenal medulla and the synaptic vesicles of postganglionic sympathetic neurons(PMID:11857564).The DBH is glycosylated (63--67 kDa) while still in the membrane(PMID:2325165) and it can form a dimer(PMID:8546710).

Publications

Species	Application	Title
mouse	WB	Int J Mol Sci; Intermittent Hypoxia Increased the Expression of DBH and PNMT in Neuroblastoma Cells via MicroRNA-375-Mediated Mechanism.; Authors - Shin Takasawa
human	WB	Peptides; Markers of the sympathetic, parasympathetic and sensory nervous system are altered in the human diabetic choroid.; Authors - Yuan-Jun Qin
mouse	IF	Tissue Cell; Autonomic nervous system imbalance in diabetic mouse choroids; Authors - Yuan-Jun Qin

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