Anticorps Polyclonal de lapin anti-DCLK1

• Phare
• Validé par KD/KO

DCLK1 Polyclonal Antibody for IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

10

N° de cat : 21699-1-AP

Synonymes

DCAMKL1, DCDC3A, DCLK, DCLK1, doublecortin like kinase 1, KIAA0369

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Galerie de données de validation

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  WB analysis of rat brain using 21699-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 21699-1-AP (DCLK1 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 21699-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 21699-1-AP (DCLK1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 21699-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 21699-1-AP (DCLK1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IP experiment of rat brain using 21699-1-AP

  IP Result of anti-DCLK1 (IP:21699-1-AP, 4ug; Detection:21699-1-AP 1:1000) with rat brain tissue lysate 4000ug.

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Applications testées

• Résultats positifs en WB: tissu cérébral de rat, tissu cérébral de souris, tissu cérébral humain
• Résultats positifs en IP: tissu cérébral de rat

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 7 publications below
• IHC: See 1 publications below
• IF: See 4 publications below
• IP: See 1 publications below
• FC: See 1 publications below

Informations sur le produit

21699-1-AP cible DCLK1 dans les applications de WB, IP, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: DCLK1 Protéine recombinante Ag16292
• Nom complet: doublecortin-like kinase 1
• Masse moléculaire calculée: 729 aa, 81 kDa
• Poids moléculaire observé: 48 kDa, 82 kDa
• Numéro d’acquisition GenBank: BC152456
• Symbole du gène: DCLK1
• Identification du gène (NCBI): 9201
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

DCLK1 (Serine/threonine-protein kinase DCLK1) is also named as DCAMKL1, DCDC3A, KIAA0369 and belongs to the CAMK Ser/Thr protein kinase family. It is a microtubule-associated kinase that can undergo autophosphorylation and it also has microtubule-polymerizing activity that is independent of its protein kinase activity (PMID: 11124993). It plays a unique role in mitotic spindle integrity during early neurogenesis in radial glial cell proliferation and their radial process stability. DCLK1 is a unique marker for distinguishing tumor stem cells from intestinal normal stem cells (PMID: 23202126). This protein has 4 isoforms produced by alternative splicing with the molecular weight of 82 kDa, 81 kDa, 47 kDa and 48 kDa.

Publications

Species	Application	Title
mouse	IF,FC	Immunity; Intestinal Tuft-2 cells exert antimicrobial immunity via sensing bacterial metabolite N-undecanoylglycine.; Authors - Zhen Xiong
mouse	IF	Redox Biol; FUT2-dependent fucosylation of HYOU1 protects intestinal stem cells against inflammatory injury by regulating unfolded protein response; Authors - Zhe Wang
human	WB,IHC,IF,IP	Clin Transl Med; Targeting DCLK1 overcomes 5-fluorouracil resistance in colorectal cancer through inhibiting CCAR1/β-catenin pathway-mediated cancer stemness.; Authors - Lanqing Wang ; KD Validated
human	WB	J Gastroenterol; High-throughput screening identified miR-7-2-3p and miR-29c-3p as metastasis suppressors in gallbladder carcinoma.; Authors - Kai Lu ; KD Validated
human	WB	Front Pharmacol; XMD-17-51 Inhibits DCLK1 Kinase and Prevents Lung Cancer Progression.; Authors - Wei-Qiang Yang ; KD Validated
human	WB	Cell Physiol Biochem; MicroRNA-195 Suppresses the Progression of Pancreatic Cancer by Targeting DCLK1.; Authors - Bin Zhou