Anticorps Polyclonal de lapin anti-DCLK1

• Phare
• Validé par KD/KO

DCLK1 Polyclonal Antibody for WB, IHC, IF-P, IF-Fro, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF-P, IF-Fro, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications(13)

N° de cat : 21699-1-AP

Synonymes

DCAMKL1, DCDC3A, Doublecortin domain-containing protein 3A, Doublecortin-like and CAM kinase-like 1, Doublecortin-like kinase 1

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Galerie de données de validation

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  WB analysis of rat brain using 21699-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 21699-1-AP (DCLK1 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 21699-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 21699-1-AP (DCLK1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 21699-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 21699-1-AP (DCLK1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of rat brain using 21699-1-AP

  IP result of anti-DCLK1 (IP:21699-1-AP, 4ug; Detection:21699-1-AP 1:1000) with rat brain tissue lysate 4000ug.

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  IHC staining of human pancreas cancer using 21699-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 21699-1-AP (DCLK1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 21699-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 21699-1-AP (DCLK1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 21699-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 21699-1-AP (DCLK1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse brain using 21699-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using DCLK1 antibody (21699-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of mouse brain using 21699-1-AP

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse brain tissue using DCLK1 antibody (21699-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• 

  IF Staining of rat brain using 21699-1-AP

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded rat brain tissue using DCLK1 antibody (21699-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Applications testées

• Résultats positifs en WB: tissu cérébral de rat, tissu cérébral de souris, tissu cérébral humain
• Résultats positifs en IP: tissu cérébral de rat
• Résultats positifs en IHC: tissu de cancer du pancréas humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu cérébral de souris,
• Résultats positifs en IF-Fro: tissu cérébral de rat, tissu cérébral de souris

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)-FRO: IF-FRO : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 8 publications below
• IHC: See 3 publications below
• IF: See 5 publications below
• IP: See 2 publications below
• CoIP: See 1 publications below

Informations sur le produit

21699-1-AP cible DCLK1 dans les applications de WB, IHC, IF-P, IF-Fro, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: DCLK1 Protéine recombinante Ag16292
• Nom complet: doublecortin-like kinase 1
• Masse moléculaire calculée: 729 aa, 81 kDa
• Poids moléculaire observé: 48 kDa, 82 kDa
• Numéro d’acquisition GenBank: BC152456
• Symbole du gène: DCLK1
• Identification du gène (NCBI): 9201
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

DCLK1 (Serine/threonine-protein kinase DCLK1) is also named as DCAMKL1, DCDC3A, KIAA0369 and belongs to the CAMK Ser/Thr protein kinase family. It is a microtubule-associated kinase that can undergo autophosphorylation and it also has microtubule-polymerizing activity that is independent of its protein kinase activity (PMID: 11124993). It plays a unique role in mitotic spindle integrity during early neurogenesis in radial glial cell proliferation and their radial process stability. DCLK1 is a unique marker for distinguishing tumor stem cells from intestinal normal stem cells (PMID: 23202126). This protein has 4 isoforms produced by alternative splicing with the molecular weight of 82 kDa, 81 kDa, 47 kDa and 48 kDa.

Publications

Species	Application	Title
mouse	IF	Immunity; Intestinal Tuft-2 cells exert antimicrobial immunity via sensing bacterial metabolite N-undecanoylglycine.; Authors - Zhen Xiong
mouse	IF	Redox Biol; FUT2-dependent fucosylation of HYOU1 protects intestinal stem cells against inflammatory injury by regulating unfolded protein response; Authors - Zhe Wang
human	WB,IP,IHC,IF	Clin Transl Med; Targeting DCLK1 overcomes 5-fluorouracil resistance in colorectal cancer through inhibiting CCAR1/β-catenin pathway-mediated cancer stemness.; Authors - Lanqing Wang ; KD Validated
human	WB	J Gastroenterol; High-throughput screening identified miR-7-2-3p and miR-29c-3p as metastasis suppressors in gallbladder carcinoma.; Authors - Kai Lu ; KD Validated
human	WB	Front Pharmacol; XMD-17-51 Inhibits DCLK1 Kinase and Prevents Lung Cancer Progression.; Authors - Wei-Qiang Yang ; KD Validated
human	WB	Cell Physiol Biochem; MicroRNA-195 Suppresses the Progression of Pancreatic Cancer by Targeting DCLK1.; Authors - Bin Zhou

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