Anticorps Polyclonal de lapin anti-DCLRE1B

DCLRE1B Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

Publications(2)

Avis (1)

N° de cat : 13203-1-AP

Synonymes

5 exonuclease Apollo, 5' exonuclease Apollo, APOLLO, Beta-lactamase DCLRE1B, DNA cross-link repair 1B protein

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Galerie de données de validation

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  WB analysis of Jurkat using 13203-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 13203-1-AP (DCLRE1B antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 13203-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 13203-1-AP (DCLRE1B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below

Informations sur le produit

13203-1-AP cible DCLRE1B dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: DCLRE1B Protéine recombinante Ag3945
• Nom complet: DNA cross-link repair 1B (PSO2 homolog, S. cerevisiae)
• Masse moléculaire calculée: 532 aa, 60 kDa
• Poids moléculaire observé: 60 kDa
• Numéro d’acquisition GenBank: BC029687
• Symbole du gène: DCLRE1B
• Identification du gène (NCBI): 64858
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Interstrand cross-links (ICLs) is an extremely cytotoxic form of DNA damage that covalently tethers the two strands of the double helix, which inhibits fundamental nuclear functions, including transcription and DNA replication [PMID:21701511]. DCLRE1B is one of several evolutionarily conserved genes involved in repair of interstrand cross-links [PMID:10848582]. Furthermore, DCLRE1B was identified as a TRF2-interacting protein and, has a role in telomere maintenance in association with the Shelterin complex, preventing non-homologous end-joining-mediated chromosome fusions protecting leading strand telomeres [PMID:20619712].

Publications

Species	Application	Title
human	WB	Nat Struct Mol Biol; Fanconi anemia DNA crosslink repair factors protect against LINE-1 retrotransposition during mouse development; Authors - Nazareno Bona
human	WB	Sci Rep; DCLRE1B as a novel prognostic biomarker associated with immune infiltration: a pancancer analysis; Authors - Mi Zou

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Daniel (Verified Customer) (02-24-2025)

Antibody does not specifically recognize DCLRE1B. A band can still be detected in DCLRE1B knockdown cells.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: RPE1

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