Anticorps Polyclonal de lapin anti-DCP1B

DCP1B Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(1)

Avis (1)

N° de cat : 13863-1-AP

Synonymes

mRNA-decapping enzyme 1B, hDcp1b, EC:3.6.1.62, DCP1

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Galerie de données de validation

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  WB analysis using 13863-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13863-1-AP (DCP1B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat spleen using 13863-1-AP

  rat spleen tissue were subjected to SDS PAGE followed by western blot with 13863-1-AP (DCP1B Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 13863-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 13863-1-AP (DCP1B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 13863-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 13863-1-AP (DCP1B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 13863-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13863-1-AP (DCP1B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 13863-1-AP

  IP result of anti-DCP1B (IP:13863-1-AP, 4ug; Detection:13863-1-AP 1:500) with HeLa cells lysate 1520ug.

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  IHC staining of human liver cancer using 13863-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 13863-1-AP (DCP1B antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human liver cancer using 13863-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 13863-1-AP (DCP1B antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HeLa using 13863-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using DCP1B antibody (13863-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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  IF Staining of A549 using 13863-1-AP

  Immunofluorescent analysis of A549 cells using 13863-1-AP (DCP1B antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu hépatique de souris, cellules HeLa, cellules Jurkat, tissu hépatique de rat, tissu hépatique humain, tissu splénique de rat
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu de cancer du foie humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa, cellules A549

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IF: See 1 publications below

Informations sur le produit

13863-1-AP cible DCP1B dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: DCP1B Protéine recombinante Ag4845
• Nom complet: DCP1 decapping enzyme homolog B (S. cerevisiae)
• Masse moléculaire calculée: 618 aa, 68 kDa
• Poids moléculaire observé: 68 kDa
• Numéro d’acquisition GenBank: BC043437
• Symbole du gène: DCP1B
• Identification du gène (NCBI): 196513
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

DCP1B belongs to DCP1 family. DCP1B may play a role in the degradation of mRNAs, both in normal mRNA turnover and in nonsense-mediated mRNA decay. It may remove the 7-methyl guanine cap structure mRNA molecules, yielding a 5'-phosphorylated mRNA fragment and 7m-GDP.  DCP1B has 2 isoforms with the molecular mass of 56 and 68 kDa. DCP1B is associated with DM-related atherogenesis (PMID:33195466).

Publications

Species	Application	Title
	IF	Int J Mol Med; LSm1 binds to the Dengue virus RNA 3' UTR and is a positive regulator of Dengue virus replication.; Authors - Yangchao Dong

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

George (Verified Customer) (08-19-2019)

Shows up well under concentration used - diffuse in unstressed tissue, forms distinct granular structures following 90 minutes 42oC heat shock. Only tested using 488 secondary antibody but showed up brightly under low power microscopy.

• Applications: Immunohistochemistry,
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Cortical Slice Culture

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