Anticorps Polyclonal de lapin anti-DCX

• Phare
• Validé par KD/KO

DCX Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IF-Fro, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF/ICC, IF-P, IF-Fro, ELISA

Conjugaison

Non conjugué

Publications(46)

Avis (3)

N° de cat : 13925-1-AP

Synonymes

doublecortin, Doublin, Lissencephalin-X, Lis-X

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Galerie de données de validation

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  WB analysis using 13925-1-AP

  Fetal human brain lysates were subjected to SDS PAGE followed by western blot with 13925-1-AP (DCX antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 13925-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13925-1-AP (DCX antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of SH-SY5Y using 13925-1-AP

  WB result of DCX antibody (13925-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DCX transfected SH-SY5Y cells.

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  IHC staining of human gliomas using 13925-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 13925-1-AP (DCX antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat brain using 13925-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 13925-1-AP (DCX antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 13925-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13925-1-AP (DCX antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse brain using 13925-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using DCX antibody (13925-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse brain using 13925-1-AP

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse brain tissue using DCX antibody (13925-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  IF Staining of SH-SY5Y using 13925-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using DCX antibody (13925-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules SH-SY5Y, tissu cérébral humain fœtal
• Résultats positifs en IHC: tissu de gliome humain, tissu cérébral de rat, tissu cérébral de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu cérébral de souris,
• Résultats positifs en IF-Fro: tissu cérébral de souris,
• Résultats positifs en IF/ICC: cellules SH-SY5Y,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:12000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)-FRO: IF-FRO : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 12 publications below
• IHC: See 1 publications below
• IF: See 39 publications below

Informations sur le produit

13925-1-AP cible DCX dans les applications de WB, IHC, IF/ICC, IF-P, IF-Fro, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris, Hamster
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: DCX Protéine recombinante Ag4945
• Nom complet: doublecortin
• Masse moléculaire calculée: 402 aa, 45 kDa
• Poids moléculaire observé: 40 kDa
• Numéro d’acquisition GenBank: BC027925
• Symbole du gène: DCX
• Identification du gène (NCBI): 1641
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

DCX (Doublecortin) is a microtubule-associated protein that is essential for normal brain development. DCX is expressed in migrating neurons throughout the central and peripheral nervous system during embryonic and postnatal development. Anti-DCX antibodies are widely used for detection of neurogenesis. It's also a common neuron marker since its expression is specific to neurons.

Publications

Species	Application	Title
human	WB,IF	Nat Commun; m6a methylation orchestrates IMP1 regulation of microtubules during human neuronal differentiation; Authors - Pierre Klein
mouse	WB	Nat Commun; Ten-eleven translocation 1 mediated-DNA hydroxymethylation is required for myelination and remyelination in the mouse brain.; Authors - Ming Zhang
mouse	IF	Phytomedicine; Ginsenoside Rg1 enriches gut microbial indole-3-acetic acid to alleviate depression-like behavior in mice via oxytocin signaling; Authors - Siqi Yu
human	WB	PLoS Pathog; Human cytomegalovirus pUL97 upregulates SOCS3 expression via transcription factor RFX7 in neural progenitor cells; Authors - Xian-Zhang Wang
mouse	IF	Brain Behav Immun; Bacteroides species differentially modulate depression-like behavior via gut-brain metabolic signaling.; Authors - Youying Zhang
mouse	IF	J Neuroinflammation; Exosomes derived from bone-marrow mesenchymal stem cells alleviate cognitive decline in AD-like mice by improving BDNF-related neuropathology.; Authors - Sen Liu

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Sampath (Verified Customer) (12-02-2019)

It worked very well brain samples and gave me a strong signal for DCX at 1:1000 dilution for western blots. I had to increase the dilution to 1:2000. This is the antibody I was looking for. If you are looking for DCX antibody, I will recommend this.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000

FH

Sheed (Verified Customer) (01-26-2019)

Works well with Edu click reaction

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Mouse

FH

Eleonora (Verified Customer) (12-11-2018)

The antibody works well, but it has too much interaction with the protein ladder (I have used Fisher BioReagent EZ-RUN).

• Applications: Western Blot,
• Primary Antibody Dilution: 1:600
• Cell Tissue Type: cortex (right) and hippocampus (left) mouse 3 weeks

DCX Antibody Western Blot, validation (1:600 dilution) in cortex (right) and hippocampus (left) mouse 3 weeks (Cat no:13925-1-AP)

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