Anticorps Polyclonal de lapin anti-DDX21

• Phare
• Validé par KD/KO

DDX21 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, chIP, RIP, ELISA

Conjugaison

Non conjugué

Publications(42)

Avis (2)

N° de cat : 10528-1-AP

Synonymes

Gu-alpha, GUA, Gu alpha, EC:3.6.4.13, DExD-box helicase 21

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Galerie de données de validation

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  WB analysis using 10528-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10528-1-AP (DDX21 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10528-1-AP

  WB result of DDX21 antibody (10528-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DDX21 transfected HeLa cells.

• 

  WB analysis using 10528-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10528-1-AP (DDX21 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10528-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10528-1-AP (DDX21 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of COLO 320 using 10528-1-AP

  IP result of anti-DDX21 (IP:10528-1-AP, 4ug; Detection:10528-1-AP 1:1000) with COLO 320 cells lysate 2800ug.

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  IP experiment of COLO 320 using 10528-1-AP

  IP result of anti-DDX21 (IP:10528-1-AP, 4ug; Detection:10528-1-AP 1:15000) with COLO 320 cells lysate 2400 ug.

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  IHC staining of human colon cancer using 10528-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10528-1-AP (DDX21 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 10528-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10528-1-AP (DDX21 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 10528-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10528-1-AP (DDX21 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 10528-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10528-1-AP (DDX21 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 10528-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using DDX21 antibody (10528-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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  IF Staining of HeLa using 10528-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using DDX21 antibody (10528-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  FC experiment of HepG2 using 10528-1-AP

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human DDX21 (10528-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Applications testées

• Résultats positifs en WB: cellules COLO 320, cellules HeLa, cellules HepG2, cellules Jurkat, cellules PC-3
• Résultats positifs en IP: cellules COLO 320,
• Résultats positifs en IHC: tissu de cancer du côlon humain, tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,
• Résultats positifs en FC (Intra): cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 9 publications below
• WB: See 34 publications below
• IHC: See 6 publications below
• IF: See 11 publications below
• IP: See 2 publications below
• CoIP: See 3 publications below
• ChIP: See 1 publications below
• RIP: See 2 publications below

Informations sur le produit

10528-1-AP cible DDX21 dans les applications de WB, IHC, IF/ICC, FC (Intra), IP, CoIP, chIP, RIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: DDX21 Protéine recombinante Ag0804
• Nom complet: DEAD (Asp-Glu-Ala-Asp) box polypeptide 21
• Masse moléculaire calculée: 87 kDa
• Poids moléculaire observé: 87 kDa
• Numéro d’acquisition GenBank: BC008071
• Symbole du gène: DDX21
• Identification du gène (NCBI): 9188
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

DX21 protein belongs to DEAD box protein family which is characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD). As a putative RNA helicase, DDX21 unwinds double-stranded RNA, folds single-stranded RNA and is involved in process including ribosomal RNA biogeneis, RNA editing and general transcription. Interaction of DDX21 and c-Jun was reported in ribosomal RNA processing. DDX21 exists as two isoforms, molecular weight of modified isoform one is about 87 -100 kDa, and the post-modified isoform is about 75-85 kDa. Catalog# 10528-1-AP is a rabbit polyclonal antibody raised against N-terminal of human DDX21.

Publications

Species	Application	Title
	CoIP	Science; lncRNA SLERT controls phase separation of FC/DFCs to facilitate Pol I transcription.; Authors - Man Wu
human	WB,IF,chIP	Cell; SLERT Regulates DDX21 Rings Associated with Pol I Transcription.; Authors - Yu-Hang Xing ; KO Validated
human	WB,IHC,IF	Mol Cell; Activation of PARP-1 by snoRNAs Controls Ribosome Biogenesis and Cell Growth via the RNA Helicase DDX21.; Authors - Dae-Seok Kim
human	WB	Nucleic Acids Res; GPATCH4 functions as a regulator of nucleolar R-loops in hepatocellular carcinoma cells; Authors - Yi-Ming Zhao
mouse	WB	Dev Cell; DDX20 is required for cell-cycle reentry of prospermatogonia and establishment of spermatogonial stem cell pool during testicular development in mice; Authors - Dingfeng Zou
human	IP	Dev Cell; PRL3-DDX21 Transcriptional Control of Endolysosomal Genes Restricts Melanocyte Stem Cell Differentiation.; Authors - Jeanette A Johansson

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Alexandra (Verified Customer) (09-04-2023)

Very good signal. I would recommend a much higher dilution than 1:200.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: P19 derived neurons

DDX21 Antibody Immunofluorescence validation (1:200 dilution) in P19 derived neurons (Cat no:10528-1-AP)

FH

Wael (Verified Customer) (12-13-2022)

Hek293 fractionated to nuclear and cytoplasmic fractions, DDX21 antibody detects strong band at the expected size of DDX21 and showing nuclear localization as expected

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Hek293

DDX21 Antibody Western Blot validation (1:1000 dilution) in Hek293 (Cat no:10528-1-AP)

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