Anticorps Polyclonal de lapin anti-DDX3

• Phare
• Validé par KD/KO

DDX3 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, RIP, IP, IHC, IF, FC, CoIP, ELISA

Conjugaison

Non conjugué

Publications

24

Avis (1)

N° de cat : 11115-1-AP

Synonymes

DBX, DDX14, DDX3, DDX3X, DEAD box, X isoform, Helicase like protein 2, HLP2

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Galerie de données de validation

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  WB analysis using 11115-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11115-1-AP (DDX3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 11115-1-AP

  WB result of DDX3 antibody (11115-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DDX3 transfected HepG2 cells.

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  WB analysis using 11115-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11115-1-AP (DDX3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 11115-1-AP

  WB result of DDX3 antibody (11115-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DDX3 transfected HeLa cells.

• 

  WB analysis using 11115-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11115-1-AP (DDX3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 11115-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 11115-1-AP (DDX3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HT-1080 using 11115-1-AP

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 11115-1-AP (DDX3 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 11115-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 11115-1-AP (DDX3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 11115-1-AP

  IP Result of anti-DDX3 (IP:11115-1-AP, 4ug; Detection:11115-1-AP 1:300) with HeLa cells lysate 2480ug.

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  IHC staining of human malignant melanoma using 11115-1-AP

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 11115-1-AP (DDX3 antibody) at dilution of 1:200 (under 40x lens)..

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  IHC staining of human malignant melanoma using 11115-1-AP

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 11115-1-AP (DDX3 antibody) at dilution of 1:200 (under 10x lens)..

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  IHC staining of human cervical cancer using 11115-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 11115-1-AP (DDX3 antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of HepG2 using 11115-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using DDX3 antibody (11115-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HepG2 using 11115-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using DDX3 antibody (11115-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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  IF Staining of HepG2 using 11115-1-AP

  Immunofluorescent analysis of HepG2 cells using 11115-1-AP (DDX3 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

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  FC experiment of HeLa using 11115-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human DDX3 (11115-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules C6, cellules HepG2, cellules HT-1080, cellules K-562, cellules NIH/3T3, cellules PC-12, tissu cérébral de rat, tissu cérébral de souris, tissu hépatique de souris
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu de mélanome malin humain, tissu de cancer du col de l'utérus humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2,
• Résultats positifs en cytométrie: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:200-1:800
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 7 publications below
• WB: See 22 publications below
• IHC: See 4 publications below
• IF: See 7 publications below
• CoIP: See 4 publications below
• RIP: See 2 publications below

Informations sur le produit

11115-1-AP cible DDX3 dans les applications de WB, RIP, IP, IHC, IF, FC, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: DDX3 Protéine recombinante Ag1614
• Nom complet: DEAD (Asp-Glu-Ala-Asp) box polypeptide 3, X-linked
• Masse moléculaire calculée: 73 kDa
• Poids moléculaire observé: 73 kDa
• Numéro d’acquisition GenBank: BC011819
• Symbole du gène: DDX3X
• Identification du gène (NCBI): 1654
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

DDX3X, also named as DBX, DDX3 and HLP2, belongs to the DEAD box helicase family and DDX3/DED1 subfamily. It is ATP-dependent RNA helicase. DDX3X acts as a cofactor for XPO1-mediated nuclear export of incompletely spliced HIV-1 Rev RNAs. It is also involved in HIV-1 replication. DDX3X interacts specifically with hepatitis C virus core protein resulting in a change in intracellular location. 73 kDa is the target band. 60-65 kDa is some other isoform. This antibody can bind both DDX3X and DDX3Y for the close sequences.

Publications

Species	Application	Title
	IF	Neuron; Pathogenic DDX3X Mutations Impair RNA Metabolism and Neurogenesis during Fetal Cortical Development.; Authors - Ashley L Lennox
human	WB	Neuron; CRISPR-Cas9 Screens Identify the RNA Helicase DDX3X as a Repressor of C9ORF72 (GGGGCC)n Repeat-Associated Non-AUG Translation.; Authors - Weiwei Cheng ; KD Validated
human	WB	Pharmacol Res; HPV E7-drived ALKBH5 promotes cervical cancer progression by modulating m6A modification of PAK5; Authors - Fu-Chun Huo ; KD Validated
mouse	WB,IF,CoIP	Ecotoxicol Environ Saf; Aluminum exposure induces central nervous system impairment via activating NLRP3-medicated pyroptosis pathway; Authors - Wudi Hao
	WB,IF	J Virol; DDX3X Is Hijacked by Snakehead Vesiculovirus Phosphoprotein To Facilitate Virus Replication via Stabilization of the Phosphoprotein; Authors - Congran Bei
human	WB,IF	Oncotarget; (DEAD)-box RNA helicase 3 modulates NF-κB signal pathway by controlling the phosphorylation of PP2A-C subunit.; Authors - Xin Wang ; KD Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Shannon (Verified Customer) (07-19-2022)

Worked well, also binds to different isoforms/protein dimers

• Applications: Western Blot, Immunoprecipitation
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: Jurkat T-Cells

DDX3 Antibody Western Blot,Immunoprecipitation validation (1:2000 dilution) in Jurkat T-Cells (Cat no:11115-1-AP)