Anticorps Polyclonal de lapin anti-DDX46
DDX46 Polyclonal Antibody for WB, IHC, IP, ELISA
Hôte / Isotype
Lapin / IgG
Réactivité testée
Humain, rat, souris
Applications
WB, IHC, IF, IP, ELISA
Conjugaison
Non conjugué
N° de cat : 16927-1-AP
Synonymes
Galerie de données de validation
Applications testées
| Résultats positifs en WB | cellules HEK-293, cellules HeLa, cellules NIH/3T3 | 
| Résultats positifs en IP | cellules HEK-293, | 
| Résultats positifs en IHC | tissu d'estomac humain, human ovary cancer tissue il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0. | 
Dilution recommandée
| Application | Dilution | 
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 | 
| Immunoprécipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate | 
| Immunohistochimie (IHC) | IHC : 1:50-1:500 | 
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Applications publiées
| WB | See 2 publications below | 
| IF | See 2 publications below | 
Informations sur le produit
16927-1-AP cible DDX46 dans les applications de WB, IHC, IF, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris
| Réactivité | Humain, rat, souris | 
| Réactivité citée | Humain | 
| Hôte / Isotype | Lapin / IgG | 
| Clonalité | Polyclonal | 
| Type | Anticorps | 
| Immunogène | DDX46 Protéine recombinante Ag10342 | 
| Nom complet | DEAD (Asp-Glu-Ala-Asp) box polypeptide 46 | 
| Masse moléculaire calculée | 1031 aa, 117 kDa | 
| Poids moléculaire observé | 120-125 kDa | 
| Numéro d’acquisition GenBank | BC012304 | 
| Symbole du gène | DDX46 | 
| Identification du gène (NCBI) | 9879 | 
| Conjugaison | Non conjugué | 
| Forme | Liquide | 
| Méthode de purification | Purification par affinité contre l'antigène | 
| Tampon de stockage | PBS with 0.02% sodium azide and 50% glycerol | 
| Conditions de stockage | Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA. | 
Informations générales
DDX46, also named as KIAA0801, is a 1031 amino acid protein, which belongs to the DEAD box helicase family. DDX46 has been shown to be required at the early step of pre-spliceosome assembly. It uses the energy released by the hydrolysis of ATP to rearrange local RNA-RNA or protein-RNA interactions. The nuclear DDX46 acted as a negative regulator of the antiviral innate response by recruiting the m6A'eraser' ALKBH5 to induce retention of antiviral innate mRNA in the nucleus.
Protocole
| Product Specific Protocols | |
|---|---|
| WB protocol for DDX46 antibody 16927-1-AP | Download protocol | 
| IHC protocol for DDX46 antibody 16927-1-AP | Download protocol | 
| IP protocol for DDX46 antibody 16927-1-AP | Download protocol | 
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols | 
Publications
| Species | Application | Title | 
|---|---|---|
| Elife Characterisation of the biflavonoid hinokiflavone as a pre-mRNA splicing modulator that inhibits SENP. | ||
| PLoS Pathog Epstein-Barr virus subverts mevalonate and fatty acid pathways to promote infected B-cell proliferation and survival. | ||
| Sci Rep Nuclear bodies formed by polyQ-ataxin-1 protein are liquid RNA/protein droplets with tunable dynamics. | ||
| Cell Rep Epstein-Barr virus BNRF1 destabilizes SMC5/6 cohesin complexes to evade its restriction of replication compartments. | ||
| Neoplasma Oncogenic DDX46 promotes pancreatic cancer development and gemcitabine resistance by facilitating the JMJD6/CDK4 signaling pathway | 
Avis
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
| FH Xiao (Verified Customer) (05-27-2025) | This antibody works very good for WB (HT1080, 293T cell lines), but not good for IP (HT1080, 293T cell lines) in my hand. 
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