Anticorps Polyclonal de lapin anti-DDX46

DDX46 Polyclonal Antibody for WB, IHC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, IP, ELISA

Conjugaison

Non conjugué

Publications(5)

Avis (1)

N° de cat : 16927-1-AP

Synonymes

DEAD box protein 46, EC:3.6.4.13, KIAA0801, Probable ATP-dependent RNA helicase DDX46

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Galerie de données de validation

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  WB analysis of HEK-293 using 16927-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 16927-1-AP (DDX46 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 16927-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16927-1-AP (DDX46 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 16927-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 16927-1-AP (DDX46 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 16927-1-AP

  IP result of anti-DDX46 (IP:16927-1-AP, 4ug; Detection:16927-1-AP 1:1000) with HEK-293 cells lysate 1280 ug.

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  IHC staining of human stomach using 16927-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 16927-1-AP (DDX46 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary cancer using 16927-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 16927-1-AP (DDX46 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, cellules NIH/3T3
• Résultats positifs en IP: cellules HEK-293,
• Résultats positifs en IHC: tissu d'estomac humain, human ovary cancer tissue; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IF: See 2 publications below

Informations sur le produit

16927-1-AP cible DDX46 dans les applications de WB, IHC, IF, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: DDX46 Protéine recombinante Ag10342
• Nom complet: DEAD (Asp-Glu-Ala-Asp) box polypeptide 46
• Masse moléculaire calculée: 1031 aa, 117 kDa
• Poids moléculaire observé: 120-125 kDa
• Numéro d’acquisition GenBank: BC012304
• Symbole du gène: DDX46
• Identification du gène (NCBI): 9879
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

DDX46, also named as KIAA0801, is a 1031 amino acid protein, which belongs to the DEAD box helicase family. DDX46 has been shown to be required at the early step of pre-spliceosome assembly. It uses the energy released by the hydrolysis of ATP to rearrange local RNA-RNA or protein-RNA interactions. The nuclear DDX46 acted as a negative regulator of the antiviral innate response by recruiting the m6A'eraser' ALKBH5 to induce retention of antiviral innate mRNA in the nucleus.

Publications

Species	Application	Title
human	IF	Elife; Characterisation of the biflavonoid hinokiflavone as a pre-mRNA splicing modulator that inhibits SENP.; Authors - Andrea Pawellek
human	WB	PLoS Pathog; Epstein-Barr virus subverts mevalonate and fatty acid pathways to promote infected B-cell proliferation and survival.; Authors - Liang Wei Wang
human	IF	Sci Rep; Nuclear bodies formed by polyQ-ataxin-1 protein are liquid RNA/protein droplets with tunable dynamics.; Authors - Sunyuan Zhang
human	WB	Cell Rep; Epstein-Barr virus BNRF1 destabilizes SMC5/6 cohesin complexes to evade its restriction of replication compartments.; Authors - Stephanie Pei Tung Yiu
		Neoplasma; Oncogenic DDX46 promotes pancreatic cancer development and gemcitabine resistance by facilitating the JMJD6/CDK4 signaling pathway; Authors - Guang Yang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Xiao (Verified Customer) (05-27-2025)

This antibody works very good for WB (HT1080, 293T cell lines), but not good for IP (HT1080, 293T cell lines) in my hand.

• Applications: Western Blot, Immunoprecipitation
• Primary Antibody Dilution: 1:2000 for western blot

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