Anticorps Recombinant de lapin anti-DDX60

DDX60 Recombinant Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

230293C10

N° de cat : 82969-1-RR

Synonymes

230293C10, DEAD box protein 60, EC:3.6.4.13, Probable ATP-dependent RNA helicase DDX60

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Galerie de données de validation

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  WB analysis using 82969-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82969-1-RR (DDX60 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach using 82969-1-RR

  Immunohistochemical analysis of paraffin-embedded human normal stomach slide using 82969-1-RR (DDX60 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human stomach using 82969-1-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human stomach tissue using DDX60 antibody (82969-1-RR, Clone: 230293C10 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human stomach using 82969-1-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human stomach tissue using DDX60 antibody (82969-1-RR, Clone: 230293C10 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A431 using 82969-1-RR

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using DDX60 antibody (82969-1-RR, Clone: 230293C10 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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  FC experiment of A431 using 82969-1-RR

  1x10^6 A431 cells were intracellularly stained with 0.2 ug DDX60 Recombinant antibody (82969-1-RR, Clone:230293C10) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.2 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 82969-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82969-1-RR against Human DDX60 were performed. The affinity constant is 0.145 nM.

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Applications testées

• Résultats positifs en WB: cellules A549, cellules HEK-293, cellules MG U-87, cellules U-251
• Résultats positifs en IHC: tissu d'estomac humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu d'estomac humain,
• Résultats positifs en IF/ICC: cellules A431,
• Résultats positifs en FC (Intra): cellules A431,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF)-P: IF-P : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

82969-1-RR cible DDX60 dans les applications de WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: DDX60 Protéine recombinante Ag34406
• Nom complet: DEAD (Asp-Glu-Ala-Asp) box polypeptide 60
• Masse moléculaire calculée: 1712 aa, 198 kDa
• Poids moléculaire observé: 170-200 kDa
• Numéro d’acquisition GenBank: BC038115
• Symbole du gène: DDX60
• Identification du gène (NCBI): 55601
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

DDX60, also called FLJ20035, is an IFN-inducible gene that has been identified via a microarray analysis of genes induced by viral infection in human dendritic cells (DCs). DDX60 expression correlated strongly with immune checkpoint and immune system-related metagene clusters, and DDX60 promoted cell proliferation, migration, and invasion and was related to poor prognosis and immune resistance(PMID: 37274827). Involved in RIG-I-dependent and independent innate immune responses, DDX60 has been proven to be associated with the development of tumors.

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