Anticorps Polyclonal de lapin anti-DEFA6

DEFA6 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications(2)

Avis (1)

N° de cat : 17923-1-AP

Synonymes

DEF6, Defensin 6, Defensin, alpha 6, Defensin-6, HD 6

---

Galerie de données de validation

• 

  WB analysis of COLO 320 using 17923-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 17923-1-AP (DEFA6 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. (10 Sec exposure).

• 

  WB analysis of mouse small intestine using 17923-1-AP

  mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 17923-1-AP (DEFA6 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human small intestine using 17923-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 17923-1-AP (DEFA6 Antibody) at dilution of 1:400 (under 10x lens).

• 

  IHC staining of human small intestine using 17923-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 17923-1-AP (DEFA6 Antibody) at dilution of 1:400 (under 40x lens).

• 

  IHC staining of human small intestine using 17923-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 17923-1-AP (DEFA6 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human small intestine using 17923-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 17923-1-AP (DEFA6 Antibody) at dilution of 1:200 (under 40x lens).

---

Applications testées

• Résultats positifs en WB: cellules COLO 320, tissu d'intestin grêle de souris
• Résultats positifs en IHC: tissu d'intestin grêle humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IF: See 2 publications below

Informations sur le produit

17923-1-AP cible DEFA6 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: DEFA6 Protéine recombinante Ag12341
• Nom complet: defensin, alpha 6, Paneth cell-specific
• Masse moléculaire calculée: 100 aa, 11 kDa
• Poids moléculaire observé: 6-10 kDa
• Numéro d’acquisition GenBank: BC093951
• Symbole du gène: DEFA6
• Identification du gène (NCBI): 1671
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Defensins are a family of antimicrobial and cytotoxic peptides thought to be involved in host defense. DEFA6 is alpha 6 of Defensin. This protein is secret expression and can be cleaved in to the fragments with MW 4-6 kDa. It can be used as a maker of Paneth cell. DEFA6 has very low antimicrobial activity against Gram-negative and Gram-positive bacteria. it may protect cells against infection with HIV-1.

Publications

Species	Application	Title
Human	IF	J Clin Invest; Human intestinal bitter taste receptors regulate innate immune responses and metabolic regulators in obesity.; Authors - Kathrin I Liszt
human	IF	Mol Metab; Bitter-tasting drugs tune GDF15 and GLP-1 expression via bitter taste or motilin receptors in the intestine of patients with obesity; Authors - Qian Wang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Md Zahirul (Verified Customer) (07-23-2025)

For WB, 4hr incubation also showed the same intensity of results as overnight.

• Applications: Western Blot, Immunohistochemistry
• Primary Antibody Dilution: 1:1000 for WB, 1:250 for IHC
• Cell Tissue Type: Colorectal Cancer Cells and Patient Derived Organoid

{{ptg:RelatedPrimaryAntibodies}}